B6;129S6-Sp140tm1Ptsn/Kctt
Status | Available to order |
EMMA ID | EM:09496 |
International strain name | B6;129S6-Sp140tm1Ptsn/Kctt |
Alternative name | B6;129S6-Sp140-/- |
Strain type | Targeted Mutant Strains : Knock-out |
Allele/Transgene symbol | Sp140tm1.1Ptsn |
Gene/Transgene symbol | Sp140 |
Information from provider
Provider | Mario Saare |
Provider affiliation | Molecular Pathology group, Institute of Biomedicine and Translational Medicine, University of Tartu |
Genetic information | The ES cells derived from 129S6/SvEvTac mouse strain were electroporated with a vector containing the neo and TK cassettes for selection, and loxP1 sites that were used to cut out the neo cassette together with exon 9 and partially exon 10. The cloned ES cells were transferred to C57BL/6J blastocysts. |
Phenotypic information | Sp140 is strongly expressed in B cells. We anticipated the knock-out to have an effect on the levels of different immunoglobulin classes, but the antibody production was unaltered. Also, we tested the avidity of ovalbumin-specific antibodies, but no changes could be determined. B cell population numbers and proportions were normal. The animals are fertile and age normally. |
Breeding history | The strain has not been backcrossed. The mice have been inbred for five generations. |
References | None available |
Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | Karolinska Institutet, Stockholm, Sweden |
Animals used for archiving | heterozygous C57BL/6J |
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