Sperm Freezing With L Glutamine
Cryopreservation Protocols
All EMMA partners use validated protocols for mouse embryo and spermatozoa freezing techniques, in vitro fertilisation procedures and embryo transfers. However, the EMMA partners use different protocols that are optimized for their individual settings. EMMA distribution centres will provide to customers ordering frozen embryos or spermatozoa the centre specific validated thawing protocols. The protocols provided for download below are practiced in EMMA training courses at the Mary Lyon Centre at MRC Harwell and are routinely used by the Harwell EMMA node.
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Training Videos
The Harwell node provides a pair of videos demonstrating a technique for embryo vitrification in straws that is used at the Mary Lyon Centre. The short films take you step-by-step through the process of freezing mouse embryos and can be viewed on the Mary Lyon Centre YouTube channel.
Embryo vitrification in straws using DAP213 method
Warming procedure for embryo vitrification in straws
Shipment and storage of mouse sperm on dry ice
Cryopreservation and IVF Protocols
Sperm Freezing Protocol For Epididymides After Refrigerated Shipment
Vitrification Of Embryos And Thawing Vitrified Embryos
Controlled Rate Embryo Freezing And Thawing
Embryo Harvesting
Culturing Embryos
Embryo Transfer Oviduct
TCET Non Surgical Embryo Transfer
IVF Freshly Harvested Sperm
IVF Frozen Sperm Sample (CPA Prepared With L Glutamine)
IVF Frozen Sperm Sample (MTG)
IVF Frozen Sperm Samples (Vials)
IVF Protocol Fresh Sperm From Epididymides Following Refrigerated Transport
Laser Assisted IVF
Superovulation For Oocyte Recovery
Oocyte Harvest For Vitrification
Acid Tyrodes Treatment Of Oocytes
Blastocyst Genotyping
CRE Treatment Of Embryos
Embryo And Adult LacZ Wholemount Protocol
Shipment Protocols
EMMA partners have developed and implemented innovative shipment protocols that facilitate fast and flexible exchange processes, reduce costs and provide alternatives to live animal transportation.
Selected References
Consumables
Aliquoting And Preparation Of 0.25mM & 1.0mM Reduced Glutathione (GSH)
Aliquoting And Testing Of Soluble CRE Recombinase Enzyme
Consumables List
Preparation And Storage Of Analgesic Agents
Preparation And Use Of Hyaluronidase
Preparation Of CPA With L Glutamine
Preparation Of High Calcium HTF Medium For IVF
Preparation Of Hormones For Superovulation
Preparation Of KSOM
Preparation Of Lifor
Preparation Of Propolyene Glycol And Sucrose For Controlled Rate Freezing
Preparation Of TYH & MBCD
Preparing JM8 BL6+LIF Media
Preparing M2
Preparing Solutions For Vitrification