MGI phenotypes (allele matching)
- decreased incidence of tumors by chemical induction / MGI
B6.129P2-Hipk1tm1Mak/Cnbc
| Status | Available to order |
| EMMA ID | EM:07222 |
| International strain name | B6.129P2-Hipk1tm1Mak/Cnbc |
| Alternative name | P53bk / HIPK1 (Hipk1 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Hipk1tm1Mak |
| Gene/Transgene symbol | Hipk1 |
Information from provider
| Provider | Tak W Mak |
| Provider affiliation | Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute |
| Genetic information | Targeting vector replaced 2.7 kb of genomic DNA containing the first exon of Hipk1 (encoding the putative kinase domain) with a neomycin-resistance cassette inserted in sense orientation to Hipk1 transcription. |
| Phenotypic information | Gene-targeted Hipk1 -/- mice were grossly normal but oncogenically transformed; Hipk1 -/- mouse embryonic fibroblasts exhibited reduced transcription of Mdm2 and were more susceptible than transformed Hipk1 +/+ cells to apoptosis induced by DNA damage. Carcinogen-treated Hipk1 -/- mice developed fewer and smaller skin tumors than Hipk1 +/+ mice. |
| Breeding history | Pure C57BL/6, backcrossed 10 times. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6J |
Disease and phenotype information
Literature references
- Characterization of cells and gene-targeted mice deficient for the p53-binding kinase homeodomain-interacting protein kinase 1 (HIPK1).;Kondo Seiji, Lu Ying, Debbas Michael, Lin Athena W, Sarosi Ildiko, Itie Annick, Wakeham Andrew, Tuan JoAnn, Saris Chris, Elliott Gary, Ma Weili, Benchimol Samuel, Lowe Scott W, Mak Tak Wah, Thukral Sushil K, ;2003;Proceedings of the National Academy of Sciences of the United States of America;100;5431-6; 12702766