C57BL/6-Tg(PF4-RAPGEF1*)8A3Cgu/Cnbc
| Status | Available to order |
| EMMA ID | EM:06335 |
| International strain name | C57BL/6-Tg(PF4-RAPGEF1*)8A3Cgu/Cnbc |
| Alternative name | tgPF4-C3GDeltaCat |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(PF4-RAPGEF1*)8A3Cgu |
| Gene/Transgene symbol | Tg(PF4-RAPGEF1*)8A3Cgu |
Information from provider
| Provider | Carmen Guerrero |
| Provider affiliation | Medicine, Center for Cancer Research (IBMCC)-University of Salamanca |
| Genetic information | RAPGEF1 (C3G) gene with a deletion in its catalytic domain (last 457 bp of the complete cDNA) is expressed under the rat PF4 gene promoter that specifically directs the expression to megakaryocytes and platelets. The plasmid used to create the chimeras also contains loxP sequences and neo selection cassette. |
| Phenotypic information | Mice expressing C3GDeltaCat transgene in platelets show longer bleeding times and displays lower platelet activity than wild type siblings. |
| Breeding history | Animals have been inbred for at least 15 generations |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J |
Literature references
- C3G transgenic mouse models with specific expression in platelets reveal a new role for C3G in platelet clotting through its GEF activity.;Gutiérrez-Herrero Sara, Maia Vera, Gutiérrez-Berzal Javier, Calzada Nuria, Sanz María, González-Manchón Consuelo, Pericacho Miguel, Ortiz-Rivero Sara, González-Porras José R, Arechederra María, Porras Almudena, Guerrero Carmen, ;2012;Biochimica et biophysica acta;1823;1366-77; 22659131