B6;CBA-Tg(Alb-Egfr*)5044Ciem/Cnbc
Status | Available to order |
EMMA ID | EM:06073 |
International strain name | B6;CBA-Tg(Alb-Egfr*)5044Ciem/Cnbc |
Alternative name | B6;B6CBA-Tg(Alb-DeltaEGFR)5044Ciem |
Strain type | Transgenic Strains |
Allele/Transgene symbol | Tg(Alb-Egfr*)5044Ciem |
Gene/Transgene symbol | Tg(Alb-Egfr*)5044Ciem |
Information from provider
Provider | Jose Carlos Segovia |
Provider affiliation | INVESTIGACION BASICA, CIEMAT |
Genetic information | DeltaEGFR cDNA was cloned under the control of the albumin mouse locus harbored in BAC clone RP23-27P6 (chromosome 5) by recombineering techniques (up to 160Kbp of this construct were injected). Three transgenic mouse lines were produced; this description corresponds to line 5044, with 4-6 copies. These mice are, currently, in F4 generation of backcrossing to C57BL/6J. |
Phenotypic information | Very low or absent activation of Egfr upon Egf stimulus in isolated adult hepatocytes analyzed by Western blot. |
Breeding history | Founders were produced in B6CBAF2 background. At the moment, animals are in the fourth generation of backcross to C57BL/6J background. |
References | None available |
Homozygous fertile | not known |
Homozygous viable | not known |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
Animals used for archiving | heterozygous C57BL/6 x CBA, wild-type C57BL/6J |
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