B6.Cg-Tg(Alb-Egfr*)5026Ciem/Cnbc
| Status | Available to order |
| EMMA ID | EM:06072 |
| International strain name | B6.Cg-Tg(Alb-Egfr*)5026Ciem/Cnbc |
| Alternative name | B6.B6CBA-Tg(Alb-DeltaEGFR)5026Ciem |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Alb-Egfr*)5026Ciem |
| Gene/Transgene symbol | Tg(Alb-Egfr*)5026Ciem |
Information from provider
| Provider | Jose Carlos Segovia |
| Provider affiliation | INVESTIGACION BASICA, CIEMAT |
| Genetic information | DeltaEGFR cDNA was cloned under the control of the albumin mouse locus harbored in BAC clone RP23-27P6 (chrom. 5) by recombineering techniques (up to 160 kbp of this construct were injected). Three transgenic mouse lines were produced; this description corresponds to line 5026, with 10-20 copies. These mice are, currently, in the fifth generation of backcrossing to C57BL/6J. |
| Phenotypic information | Very low or absent activation of Egfr upon Egf stimulus in isolated adult hepatocytes analyzed by Western blot. Attenuation of Egfr phosphorylation in liver after partial hepatectomy detected by immunohistochemistry. |
| Breeding history | Founders were produced in B6CBAF2 background. At the moment, animals are in the fifth generation of backcross to C57BL/6J background. |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous (C57BL/6 x CBA)F2 |