STOCK Tg(Gfap-Pten*)6Rpm/Cnbc
| Status | Available to order |
| EMMA ID | EM:05237 |
| International strain name | STOCK Tg(Gfap-Pten*)6Rpm/Cnbc |
| Alternative name | GL1 GFAP-Pten qma |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Gfap-PTEN*)6Rpm |
| Gene/Transgene symbol | Tg(Gfap-PTEN*)6Rpm |
Information from provider
| Provider | RAFAEL PULIDO-MURILLO |
| Provider affiliation | LABORATORY OF MOLECULAR BIOLOGY OF CANCER, CENTRO DE INVESTIGACIÓN PRINCIPE FELIPE |
| Genetic information | Transgenic mouse in strain C57BL/6 x SJL; gene affected Pten (MGI ref. 109583); Pten is mutated in residues phosphorylated by CK2 (qma, Ser370Ala, Ser380Ala, Thr382Ala, Thr383Ala, and Ser385Ala). The subcellular localization of PTEN qma is nuclear in cell lines and favours the apoptosis. |
| Phenotypic information | The expression of the transgene does not have any phenotype in the mouse strain. |
| Breeding history | The founder was crossed with the wt strain C57BL/6 x SJL to generate the heterozygous animals. The heterozygous animals were backcrossed 6 times with brothers and sisters until we obtained the homozygous animals. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6 x SJL, homozygous C57BL/6 x SJL |
| Stage of embryos | 2-cell |
Literature references
- Nuclear localization of PTEN by a Ran-dependent mechanism enhances apoptosis: Involvement of an N-terminal nuclear localization domain and multiple nuclear exclusion motifs.;Gil Anabel, Andrés-Pons Amparo, Fernández Elena, Valiente Miguel, Torres Josema, Cervera Javier, Pulido Rafael, ;2006;Molecular biology of the cell;17;4002-13; 16807353
- Nuclear PTEN: a tale of many tails.;Gil A, Andrés-Pons A, Pulido R, ;2007;Cell death and differentiation;14;395-9; 17186024