C57BL/6NTac-Setd1atm1c(EUCOMM)Wtsi/H
| Status | Available to order |
| EMMA ID | EM:15687 |
| International strain name | C57BL/6NTac-Setd1atm1c(EUCOMM)Wtsi/H |
| Alternative name | C57BL/6NTac-Setd1a |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Setd1atm1c(EUCOMM)Wtsi |
| Gene/Transgene symbol |
Information from provider
| Provider | MRC, Medical Research Council |
| Provider affiliation | Mammalian Genetics Unit, Mary Lyon Centre at MRC Harwell |
| Genetic information | The L1L2_gt0 cassette was inserted at position 127379089 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 127380049. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). The tm1c allele was generated using Flp recombinase to excise the lacZ-neomycin cassette present in the tm1a allele. The tm1c allele still carries loxP sites available for excision by cre recombinase. |
| Phenotypic information | Homozygous:No abnormal phenotype reportedHeterozygous:No abnormal phenotype reported |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | not known |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Setd1a regulates progenitor B-cell-to-precursor B-cell development through histone H3 lysine 4 trimethylation and Ig heavy-chain rearrangement.;Tusi BK, Deng C, Salz T, Zeumer L, Li Y, So CW, Morel LM, Qiu Y, Huang S.;2015;FASEB J;29;1505-1515; 25550471