B6;129S-Tg(Csf1r-EGFP/Rpl10a)1Glass/Oulu
| Status | Under development - register interest |
| EMMA ID | EM:15591 |
| International strain name | B6;129S-Tg(Csf1r-EGFP/Rpl10a)1Glass/Oulu |
| Alternative name | B6;129S-Tg(Csf1r-EGFP)- founder line? Svo/JOlaHsd |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Csf1r-EGFP/Rpl10a)1Glass |
| Gene/Transgene symbol | Tg(Csf1r-EGFP/Rpl10a)1Glass |
Information from provider
| Provider | Minna Kaikkonen-Määttä |
| Provider affiliation | A.I. Virtanen -instituutti, University of Eastern Finland |
| Additional owner | The original cfms-L10a-EGFP strain was developed by Prof. Christopher Glass at University of California San Diego. The L10a-EGFP construct was provided by Prof. Nathaniel Heintz, Howard Hughes Medical Institute/The Rockefeller University. |
| Genetic information | The construct expressing L10alpha ribosomal subunit (Rpl10a) fused to EGFP was obtained from N. Heintz (Rockfeller U. NY). The EGFP-L10alpha construct was then cloned downstream of the c-fms promoter (Sasmono et al., Blood 2003) and the resulting vector injected into the pronuclei of fertilized oocytes. The EGFP-L10alpha fusion protein is constitutively expressed in myeloid cells. The expression of EGFPL10alpha allows to specifically immunprecipitate polysome bound mRNA in myeloid cells. |
| Phenotypic information | Homozygous:The expression of EGFPL10alpha allows to specifically immunprecipitate polysome bound mRNA in myeloid cells.Heterozygous:The expression of EGFPL10alpha allows to specifically immunprecipitate polysome bound mRNA in myeloid cells. |
| Breeding history | Transgenic mice were generated in the C57BL/6NHsd (code 044) genetic background and have been bred to C57BL/6JOlaHsd since 2013. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Oulu, Oulu, Finland |
Literature references
- 25-Hydroxycholesterol activates the integrated stress response to reprogram transcription and translation in macrophages.;Shibata Norihito, Carlin Aaron F, Spann Nathanael J, Saijo Kaoru, Morello Christopher S, McDonald Jeffrey G, Romanoski Casey E, Maurya Mano R, Kaikkonen Minna U, Lam Michael T, Crotti Andrea, Reichart Donna, Fox Jesse N, Quehenberger Oswald, Raetz Christian R H, Sullards M Cameron, Murphy Robert C, Merrill Alfred H, Brown H Alex, Dennis Edward A, Fahy Eoin, Subramaniam Shankar, Cavener Douglas R, Spector Deborah H, Russell David W, Glass Christopher K, ;2013;The Journal of biological chemistry;288;35812-23; 24189069