STOCK Igs5tm2.1(ACTB-EGFP,-tdTomato)Luo/Biat
| Status | Available to order |
| EMMA ID | EM:14696 |
| International strain name | STOCK Igs5tm2.1(ACTB-EGFP,-tdTomato)Luo/Biat |
| Alternative name | CD1;B6N-MADM-GT |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Igs5tm2.1(ACTB-EGFP,-tdTomato)Luo |
| Gene/Transgene symbol | Igs5 |
Information from provider
| Provider | Simon Hippenmeyer |
| Provider affiliation | Institute of Science and Technology Austria (IST Austria) |
| Additional owner | Prof. Liqun Luo, Stanford University, Biology Department, Stanford, USA. |
| Genetic information | The GT MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 1-273), a beta-globin intronic sequence containing an frt site and loxP-flanked neomycin resistance gene, the C-terminal portion of a red fluorescent protein (tdTomato; aa 4-1544) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. This entire GT MADM construct was inserted into the Iis5 locus locate at about 0.7 kb downstream of exon 5 of the Rps9 gene at 2.13cM. Mice were bred to Hprt-Cre mice (on an undisclosed genetic background) and the floxed neomycin resistance gene was removed. (PMID 23453967) |
| Phenotypic information | Homozygous:not availableHeterozygous:not available |
| Breeding history | Chimeras were generated by injecting C57BL/6N embryonic stem cells into BALB/cRj blastocysts and then crossed with CD1 mice. Afterwards they were intercrossed. |
| References |
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| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
Literature references
- Mosaic analysis with double markers reveals cell-type-specific paternal growth dominance.;Hippenmeyer Simon, Johnson Randy L, Luo Liqun, ;2013;Cell reports;3;960-7; 23453967
- Genetic mosaic dissection of Lis1 and Ndel1 in neuronal migration.;Hippenmeyer Simon, Youn Yong Ha, Moon Hyang Mi, Miyamichi Kazunari, Zong Hui, Wynshaw-Boris Anthony, Luo Liqun, ;2010;Neuron;68;695-709; 21092859