B6;CBA-Tln1em1Flmg/Flmg
| Status | Available to order |
| EMMA ID | EM:13074 |
| International strain name | B6;CBA-Tln1em1Flmg/Flmg |
| Alternative name | Talin1 C-mutant |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Tln1em1Flmg |
| Gene/Transgene symbol | Tln1 |
Information from provider
| Provider | Transgenics Facility B.S.R.C. |
| Provider affiliation | B.S.R.C. "Alexander Fleming" |
| Genetic information | Mice with a deletion of exons 55-57 of talin 1 gene were generated using CRISPR/Cas9 technology. Two guide RNAs were designed to target sequences outside of the selected area. A ssODN template consisting of two 60 bp fragments at each of the outer sides of the Cas9 cutting edges was used producing a deletion of 1033 bp. The CRISPR components were introduced into zygotes via the embryo electroporation method. |
| Phenotypic information | Homozygous:Embryonic lethalHeterozygous:No observable phenotype |
| Breeding history | The colony has been maintained by backcrossing talin 1 C-mutant heterozygous mice to C57BL/6J mice for six generations; talin 1 C-mutant homozygotes are embryonic lethal. |
| References | None available |
| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | heterozygous CBA x C57BL/6, wild-type C57BL/6J |
| Stage of embryos | Morula |