B6.129P2-Trp73tm2Mak/H
| Status | Available to order |
| EMMA ID | EM:13069 |
| International strain name | B6.129P2-Trp73tm2Mak/H |
| Alternative name | DNp73-/- (deltaNp73-/-) |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Trp73tm2Mak, |
| Gene/Transgene symbol | Trp73 |
Information from provider
| Provider | Gerry Melino |
| Provider affiliation | MRC Toxicology Unit |
| Additional owner | Prof. Tak Mak, University of Toronto, Canada |
| Genetic information | Mutant mice deficient for Trp73 exon 3, which is specific for DNp73 isoforms, were generated by conventional gene targeting procedures in 129/Ola embryonic stem (ES) cells. Four correctly targeted ES cell clones were selected for blastocyst injection. Blastocysts were transferred to pseudopregnant C57BL/6J female mice to generate chimaeric mice. We obtained germline transmission of the targeted allele for all four ES cell clones. Littermate offspring that were wild type or heterozygous or homozygous for the mutated DNp73 allele were created by intercrossing DNp73 +/- mice (Genes Dev., 2010 Mar 15;24(6):549-60. doi: 10.1101/gad.1873910). |
| Phenotypic information | Homozygous:Mice deficient for DNp73 are born at the normal Mendelian ratio (wild-type, 28%; heterozygous knock-out, 51%; homozygous knock-out, 20%). Both male and female DNp73-/- mice were fertile and enjoyed a normal life span. DNp73 -/- mice display neuroanatomical evidence of mild neurodegeneration. DNp73-/- mice displayed CNS atrophy. Data also suggest an involvement of DNp73 in DNA damage response (Genes Dev., 2010 Mar 15;24(6):549-60. doi: 10.1101/gad.1873910).Heterozygous:No abnormal phenotype is observed in heterozygous mice (compared to wild-type littermate) |
| Breeding history | Backcrossed for more than 10 generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Isoform-specific p73 knockout mice reveal a novel role for delta Np73 in the DNA damage response pathway.;Wilhelm Margareta T, Rufini Alessandro, Wetzel Monica K, Tsuchihara Katsuya, Inoue Satoshi, Tomasini Richard, Itie-Youten Annick, Wakeham Andrew, Arsenian-Henriksson Marie, Melino Gerry, Kaplan David R, Miller Freda D, Mak Tak W, ;2010;Genes & development;24;549-60; 20194434