IMPC phenotypes (gene matching)
B6.129P2-Pik3cbtm2.1Bvan/Cnbc
| Status | Available to order |
| EMMA ID | EM:11630 |
| International strain name | B6.129P2-Pik3cbtm2.1Bvan/Cnbc |
| Alternative name | p110beta PI3K-D931A (B6) |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Pik3cbtm2.1Bvan |
| Gene/Transgene symbol | Pik3cb |
Information from provider
| Provider | Bart Vanhaesebroeck |
| Provider affiliation | UCL Cancer Institute, University College London |
| Additional owner | the University of Edinburgh |
| Genetic information | Knock-in mice in which the endogenous Pik3cb/p110beta PI3K gene is mutated so that it now encodes a p110beta protein with the D931A mutation in the ATP binding site, converting it to a kinase-dead p110beta protein which is expressed at the same level as wild-type p110beta. These mice have been backcrossed onto the B6 background. |
| Phenotypic information | Homozygous:Mice show prenatal partial lethality at different embryonic stages (see PMID 26132308 for details). Homozygous male mice that are born are infertile with smaller than normal testicles. They cannot produce offspring.Heterozygous:Mice show reduced phenotypes of homozygous. |
| Breeding history | Backcrossed onto C57BL/6J for more than 10 generations. |
| References |
|
| Homozygous fertile | females only |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6JOlaHsd |
Disease and phenotype information
MGI phenotypes (gene matching)
- decreased body weight / MGI
- weight loss / MGI
- decreased embryo size / MGI
- postnatal growth retardation / MGI
- decreased circulating triglyceride level / MGI
- increased insulin secretion / MGI
- abnormal gluconeogenesis / MGI
- decreased lumbar vertebrae number / MGI
- decreased circulating cholesterol level / MGI
- insulin resistance / MGI
- decreased heart rate / MGI
- decreased glycogen level / MGI
- abnormal platelet physiology / MGI
- pancreatic islet hyperplasia / MGI
- decreased circulating creatinine level / MGI
- increased circulating glucose level / MGI
- increased mean corpuscular hemoglobin / MGI
- increased hemoglobin content / MGI
- increased lung weight / MGI
- pulmonary fibrosis / MGI
- decreased physiological sensitivity to xenobiotic / MGI
- decreased platelet aggregation / MGI
- decreased birth body size / MGI
- decreased sensitivity to xenobiotic induced morbidity/mortality / MGI
- embryonic lethality between somite formation and embryo turning, complete penetrance / MGI
- preweaning lethality, complete penetrance / MGI
- embryonic lethality, incomplete penetrance / MGI
- embryonic lethality before implantation, incomplete penetrance / MGI
- embryonic lethality during organogenesis, incomplete penetrance / MGI
- lethality throughout fetal growth and development, incomplete penetrance / MGI
- decreased fibroblast proliferation / MGI
Literature references
- Novel Role for p110β PI 3-Kinase in Male Fertility through Regulation of Androgen Receptor Activity in Sertoli Cells.;Guillermet-Guibert Julie, Smith Lee B, Halet Guillaume, Whitehead Maria A, Pearce Wayne, Rebourcet Diane, León Kelly, Crépieux Pascale, Nock Gemma, Strömstedt Maria, Enerback Malin, Chelala Claude, Graupera Mariona, Carroll John, Cosulich Sabina, Saunders Philippa T K, Huhtaniemi Ilpo, Vanhaesebroeck Bart, ;2015;PLoS genetics;11;e1005304; 26132308