B6.129P2-Tln1tm4.1Crit/CritH
| Status | Available to order |
| EMMA ID | EM:04999 |
| International strain name | B6.129P2-Tln1tm4.1Crit/CritH |
| Alternative name | Tln1(tm4.1Crit) |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Tln1tm4.1Crit, |
| Gene/Transgene symbol | Tln1 |
Information from provider
| Provider | Sue Monkley |
| Provider affiliation | Biomedical Services, University of Leicester |
| Additional owner | Prof David Critchley, Dept of Biochemistry, University of Leicester, Leicester, UK |
| Genetic information | Conditional talin 1 allele with loxP sites upstream of the 1st coding exon and downstream of the 4th coding exon. Cre-mediated deletion of exons 1-4 results in a null allele. |
| Phenotypic information | The homozygous floxed Tln1 mice are viable and fertile and do not exhibit evidence of any phenotype. |
| Breeding history | The line was generated with a floxed neo cassette that was deleted by crossing to cre-deleter mice. The allele was then backcrossed to C57BL/6 and then intercrossed to generate homozygous mice. The allele is maintained as homozygous. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | homozygous C57BL/6.129/Sv |
| Breeding at archiving centre | Males were archived upon arrival. No breeding was performed at the archiving centre. |
Literature references
- Talin is required for integrin-mediated platelet function in hemostasis and thrombosis.;Petrich Brian G, Marchese Patrizia, Ruggeri Zaverio M, Spiess Saskia, Weichert Rachel A M, Ye Feng, Tiedt Ralph, Skoda Radek C, Monkley Susan J, Critchley David R, Ginsberg Mark H, ;2007;The Journal of experimental medicine;204;3103-11; 18086863
- Loss of talin1 in platelets abrogates integrin activation, platelet aggregation, and thrombus formation in vitro and in vivo.;Nieswandt Bernhard, Moser Markus, Pleines Irina, Varga-Szabo David, Monkley Sue, Critchley David, Fässler Reinhard, ;2007;The Journal of experimental medicine;204;3113-8; 18086864
- Talin 1 and 2 are required for myoblast fusion, sarcomere assembly and the maintenance of myotendinous junctions.;Conti Francesco J, Monkley Sue J, Wood Malcolm R, Critchley David R, Müller Ulrich, ;2009;Development (Cambridge, England);136;3597-606; 19793892