MGI phenotypes (allele matching)
B6N-Tyrc-Brd/BrdCrCrl.FVB/N-Tg(K14-HPV38E6-E7)Tg/Tg187DKFZ/Jmar
| Status | Under development - register interest |
| EMMA ID | EM:15629 |
| International strain name | B6N-Tyrc-Brd/BrdCrCrl.FVB/N-Tg(K14-HPV38E6-E7)Tg/Tg187DKFZ/Jmar |
| Alternative name | B6N-Tyrc-Brd/BrdCrCrl.FVB/N-Tg(K14-HPV38E6-E7)Tg/Tg187DKFZ/Jmar |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(KRT14- HPV38E6E7)187Jmar |
| Gene/Transgene symbol | Tg(KRT14- HPV38E6E7)187Jmar |
Information from provider
| Provider | Jacqueline Marvel |
| Provider affiliation | Batiment Franklin, CIRI INSERM U1111 |
| Genetic information | Transgenic for the early genes E6 and E7 from the cutaneous papillomavirus type 38, under the control of the keratin 14 promoter, targeting their expression in the basal layer of epithelia. The transgene was transferred by backcross on the tyrosinase-mutated albino C57BL/6N mouse: B6N-Tyrc-Brd/BrdCrCrl. The mice are more susceptible to the development of UV-Induced tumors. The role of the immune system in this increased susceptibility is poorly understood. Hence, the backcross on C57BL/6 was performed for two reasons. First the study of the immune response in FVB mice, especially the T cells response, is relatively restricted compared to C57BL/6 where the array of tools to study T cells is very large. For example, MHC-tetramer and assorted pathogen or tumor-derived peptides allowing the follow-up of T cells dynamics and function are defined and broadly available for C57BL/6, but are not defined or available for FVB mice. Second to have access to the mutant lines that are mostly on a C57BL/6 background, having a Tg and a mutant (KO or other) on the same background simplifies the breeding strategy and reduces the number of mice used for an experiment. Reference for the original FVB/N-Tg(38E6E7)187DKFZ strain: Viarisio et al, Plos Pathogens, 2011 Jul7(7). |
| Phenotypic information | Homozygous:Basal epithelial cells express the E6 and E7 genes from HPV38 (187Tg/Tg)Heterozygous:Basal epithelial cells express the E6 and E7 genes from HPV38 (187Tg/wt) |
| Breeding history | FVB/N-Tg(38E6E7)187Dkfz mice were backcrossed 10 times to B6 albino mice obtained from Charles River (B6N-Tyrc-Brd/BrdCrCrl). The positivity for the transgene was determined by PCR. After 10 backcrosses, heterozygous mice (187Tg/wt) were intercrossed to obtain homozygous transgenic mice (187Tg/Tg). |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Temperature-sensitive oculocutaneous albinism type 1 / Orphanet_352737
- Oculocutaneous albinism type 1A / Orphanet_79431
- Oculocutaneous albinism type 1B / Orphanet_79434
- Minimal pigment oculocutaneous albinism type 1 / Orphanet_352734
- Ocular albinism with congenital sensorineural deafness / Orphanet_352740
Literature references
- E6 and E7 from beta HPV38 cooperate with ultraviolet light in the development of actinic keratosis-like lesions and squamous cell carcinoma in mice.;Viarisio Daniele, Mueller-Decker Karin, Kloz Ulrich, Aengeneyndt Birgit, Kopp-Schneider Annette, Gröne Hermann-Josef, Gheit Tarik, Flechtenmacher Christa, Gissmann Lutz, Tommasino Massimo, ;2011;PLoS pathogens;7;e1002125; 21779166