C57BL/6N-Hmgcrem1Lclem/Kctt

Status

Available to order

EMMA IDEM:14795
International strain nameC57BL/6N-Hmgcrem1Lclem/Kctt
Alternative nameC57BL/6N-Hmgcrem1Lclem/Lclem
Strain typeEndonuclease-mediated
Allele/Transgene symbolHmgcrem1Lclem
Gene/Transgene symbolHmgcr

Information from provider

ProviderHelgi Schiöth
Provider affiliationDepartment of Surgical Sciences, Functional Pharmacology and Neuroscience, Uppsala University
Genetic informationLoxP sites have been inserted around exon 5 of the Hmgcr gene by CRISPR/Cas9 mediated genome engineering. CRISPR technology details: Cas9 protein (from New England Biolabs) and gRNA were co-injected into fertilized eggs, therefore Cas9 did not integrate in the mouse genome.
Phenotypic informationHomozygous:
The mice have no pathological phenotype, since the mutation is conditional.

Heterozygous:
The mice have no pathological phenotype, since the mutation is conditional.
Breeding historyHeterozygous founders were bred with C57BL/6NTac wild-type mice to increase the colony. After this, the heterozygous mice were bred to homozigosity and inbred for 4 generations before conservation.
ReferencesNone available
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden
Animals used for archivinghomozygous 0

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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