C57BL/6N-A^tm1Brd Krt8^{tm1a(EUCOMM)Hmgu}/IcsOrl

Status	Available to order
EMMA ID	EM:09978
Citation information	RRID:IMSR_EM:09978 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-A^tm1Brd Krt8^{tm1a(EUCOMM)Hmgu}/IcsOrl
Alternative name	HEPD0592_4_D04
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Krt8^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Krt8
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	ICS, Institut Clinique de la Souris
Provider affiliation	ICS, Institut Clinique de la Souris
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0592_4_D04. For further details on the construction of this clone see the page at the IKMC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6NTac males

Disease and phenotype information

IMPC phenotypes (gene matching)

increased neutrophil cell number / IMPC
increased prepulse inhibition / IMPC
improved glucose tolerance / IMPC
decreased exploration in new environment / IMPC
preweaning lethality, complete penetrance / IMPC

MGI phenotypes (gene matching)

extramedullary hematopoiesis / MGI
abnormal intestinal epithelium morphology / MGI
rectal prolapse / MGI
abnormal colon morphology / MGI
abnormal hepatocyte morphology / MGI
enlarged spleen / MGI
decreased body weight / MGI
internal hemorrhage / MGI
hepatic necrosis / MGI
abnormal intestinal mineral absorption / MGI
abnormal placenta morphology / MGI
reduced female fertility / MGI
female infertility / MGI
chronic inflammation / MGI
hepatic steatosis / MGI
liver hemorrhage / MGI
abnormal extraembryonic tissue physiology / MGI
abnormal trophoblast layer morphology / MGI
abnormal trophoblast giant cell morphology / MGI
diarrhea / MGI
impaired placental function / MGI
decreased embryo weight / MGI
embryonic lethality during organogenesis, incomplete penetrance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Atm1Brd Krt8tm1a(EUCOMM)Hmgu/IcsOrl