MGI phenotypes (gene matching)
C57BL/6N-Snorctm1b(EUCOMM)Hmgu/Oulu
| Status | Available to order |
| EMMA ID | EM:09934 |
| International strain name | C57BL/6N-Snorctm1b(EUCOMM)Hmgu/Oulu |
| Alternative name | Tuko37F |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Snorctm1b(EUCOMM)Hmgu |
| Gene/Transgene symbol | Snorc |
Information from provider
| Provider | Anna-Marja Säämänen |
| Provider affiliation | Institute of Biomedicine/Department of Medical Biochemistry and Genetics, University of Turku |
| Genetic information | Knockdown of Snorc gene. Snorc is a novel transmembrane proteoglycan particularly expressed in cartilage tissue but also in minor amounts in some other tissues, including lactating mammary gland and prostate. For further details of this proteoglycan see: Heinonen et al. Osteoarthritis Cartilage. 2011 Aug;19(8):1026-35. An ES cell clone containing the Snorc gene targeted by a promoter driven “knockout first” EUCOMM/KOMP-CSD allele was obtained from the International Knockout Mouse Consortium (IKMC; https://www.mousephenotype.org/data/genes/MGI:1920484#order2) and used for blastocyst injection in C57BL/6NCrl mice for generation of this mouse strain. This is the 10th member of transmembrane proteoglycans and is highly conserved throughout vertebrates. In order to ensure full knock-down of Snorc, loxP flanked exons 2 and 3 were deleted from targeted Snorc tm1a allele (Snorc -) using cre/loxP site specific recombination. Male Snorc-/- (EMMA strain EM:09933) were bred with female transgenic mice, expressing cre recombinase in mature oocyte allowing recombination to occur in the zygote before 2-cell stage. Occurrence of deletion by cre/loxP recombination in offspring was determined by PCR using primers for wild-type allele and Snorc-del allele. |
| Phenotypic information | Homozygous:Delay in secondary ossification and alterations in trabecular bone structure. Long bones in adults are slightly shorter with some other alterations in the trabecular bone structure. Manuscripts to describe the phenotype are under preparation at the moment. Phenotype is almost similar to Tuko37 strain (EMMA strain EM:09933).Heterozygous:Not studied |
| Breeding history | The strain has been backcrossed 5 times to the C57BL/6NCrl strain maintained in the animal house of the University of Turku. Sibling matings have been avoided. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | University of Oulu, Oulu, Finland |
| Animals used for archiving | heterozygous C57BL/6NCrl, wild-type C57BL/6NCrl |
| Stage of embryos | 2-cell |
Disease and phenotype information
Literature references
- Snorc is a novel cartilage specific small membrane proteoglycan expressed in differentiating and articular chondrocytes.;Heinonen J, Taipaleenmäki H, Roering P, Takatalo M, Harkness L, Sandholm J, Uusitalo-Järvinen H, Kassem M, Kiviranta I, Laitala-Leinonen T, Säämänen A-M, ;2011;Osteoarthritis and cartilage;19;1026-35; 21624478
- Defects in chondrocyte maturation and secondary ossification in mouse knee joint epiphyses due to Snorc deficiency.;Heinonen J, Zhang F-P, Surmann-Schmitt C, Honkala S, Stock M, Poutanen M, Säämänen A-M, ;2017;Osteoarthritis and cartilage;25;1132-1142; 28323137