NOD.129P2(B6)-Nos2^tm1Lau/Ieg

Status	Available to order
EMMA ID	EM:09914
International strain name	NOD.129P2(B6)-Nos2^tm1Lau/Ieg
Alternative name	NOD.B6;129P2-Nos2>tm1Lau
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Nos2^tm1Lau
Gene/Transgene symbol	Nos2

Information from provider

Provider	Volker Burkart
Provider affiliation	Institut für Klinische Diabetologie, Deutsches-Diabetes-Zentrum
Genetic information	Replacement of exons 12 and 13 encoding the calmodulin-binding domain of the iNOS (Nos2) gene by a neomycin cassette.
Phenotypic information	Homozygous: After backcross on the NOD background no homozygous offspring was obtained anymore. Heterozygous: Heterozygous mice are viable and fertile and show no apparent abnormalities. No further characterization was performed.
Breeding history	B6;129P2-Nos2^tm1Lau mice, provided by Paula Sherman, were backcrossed for 32 generations on the NOD/Lt background. For the last 9 generations intercross of heterozygous mice was performed.
References	Mice lacking inducible nitric oxide synthase are not resistant to lipopolysaccharide-induced death.;Laubach V E, Shesely E G, Smithies O, Sherman P A, ;1995;Proceedings of the National Academy of Sciences of the United States of America;92;10688-92; 7479866
Homozygous fertile	no
Homozygous viable	no
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous NOD

Disease and phenotype information

IMPC phenotypes (gene matching)

decreased thigmotaxis / IMPC
abnormal eye anterior chamber depth / IMPC

MGI phenotypes (allele matching)

abnormal sleep pattern / MGI
abnormal inflammatory response / MGI
decreased inflammatory response / MGI
abnormal surfactant physiology / MGI
abnormal brain wave pattern / MGI
abnormal circadian sleep/wake cycle / MGI
decreased circulating alanine transaminase level / MGI
decreased susceptibility to injury / MGI
decreased circulating aspartate transaminase level / MGI
immune system phenotype / MGI
abnormal systemic arterial blood pressure / MGI
abnormal immune system morphology / MGI
abnormal testis morphology / MGI
decreased white adipose tissue amount / MGI
abnormal gametogenesis / MGI
abnormal reproductive system morphology / MGI
altered tumor susceptibility / MGI
increased susceptibility to bacterial infection / MGI
abnormal adaptive immunity / MGI
abnormal sperm number / MGI
increased growth rate / MGI
increased insulin sensitivity / MGI
increased testis weight / MGI
skin inflammation / MGI
abnormal male meiosis / MGI
improved glucose tolerance / MGI
abnormal enzyme/coenzyme level / MGI
increased T cell proliferation / MGI
homeostasis/metabolism phenotype / MGI
cardiovascular system phenotype / MGI
abnormal fertilization / MGI
increased circulating creatinine level / MGI
increased circulating antidiuretic hormone level / MGI
decreased circulating leptin level / MGI
enlarged seminiferous tubules / MGI
increased single-positive T cell number / MGI
enlarged inguinal lymph nodes / MGI
abnormal blood homeostasis / MGI
increased sensitivity to induced morbidity/mortality / MGI
increased food intake / MGI
increased urine pH / MGI
abnormal heart morphology / MGI
abnormal corpus callosum morphology / MGI
abnormal eating behavior / MGI
abnormal glucose homeostasis / MGI
abnormal respiratory system physiology / MGI
abnormal blood coagulation / MGI
enhanced wound healing / MGI
abnormal bone mineralization / MGI
increased susceptibility to pharmacologically induced seizures / MGI
abnormal frequency of paradoxical sleep / MGI
decreased susceptibility to induced choroidal neovascularization / MGI
abnormal physiological neovascularization / MGI
abnormal bone structure / MGI
increased left ventricle developed pressure / MGI
increased mortality induced by ionizing radiation / MGI
abnormal respiratory bronchiole morphology / MGI
abnormal osteoclast morphology / MGI
abnormal heart ventricle morphology / MGI
abnormal myocardium layer morphology / MGI
respiratory system phenotype / MGI
abnormal platelet physiology / MGI
decreased systemic arterial systolic blood pressure / MGI
decreased susceptibility to induced colitis / MGI
decreased oligodendrocyte number / MGI
mortality/aging / MGI
improved central nervous system regeneration / MGI

MGI phenotypes (gene matching)

increased bone mineral density / MGI
abnormal trabecular bone morphology / MGI
abnormal circulating glucose level / MGI
abnormal circulating serum albumin level / MGI
abnormal systemic arterial blood pressure / MGI
abnormal heart morphology / MGI
decreased digestive secretion / MGI
abnormal immune system morphology / MGI
abnormal corpus callosum morphology / MGI
abnormal uterus morphology / MGI
abnormal testis morphology / MGI
increased body weight / MGI
abnormal eating behavior / MGI
abnormal sleep pattern / MGI
abnormal osteoclast physiology / MGI
abnormal cardiovascular system physiology / MGI
increased circulating triglyceride level / MGI
abnormal digestion / MGI
abnormal homeostasis / MGI
decreased white adipose tissue amount / MGI
abnormal immune system physiology / MGI
abnormal inflammatory response / MGI
increased inflammatory response / MGI
pancreas inflammation / MGI
decreased inflammatory response / MGI
abnormal mammary gland physiology / MGI
abnormal gametogenesis / MGI
decreased litter size / MGI
abnormal motor capabilities/coordination/movement / MGI
abnormal sensory capabilities/reflexes/nociception / MGI
abnormal glucose homeostasis / MGI
premature death / MGI
abnormal cardiovascular system morphology / MGI
abnormal blood circulation / MGI
abnormal respiratory system physiology / MGI
abnormal reproductive system morphology / MGI
altered tumor susceptibility / MGI
increased susceptibility to bacterial infection / MGI
abnormal adaptive immunity / MGI
abnormal macrophage physiology / MGI
abnormal blood coagulation / MGI
abnormal eosinophil cell number / MGI
abnormal vitreous body morphology / MGI
enhanced wound healing / MGI
abnormal thermal nociception / MGI
increased systemic arterial blood pressure / MGI
increased growth rate / MGI
increased insulin sensitivity / MGI
abnormal bone mineralization / MGI
increased susceptibility to pharmacologically induced seizures / MGI
delayed wound healing / MGI
decreased circulating alanine transaminase level / MGI
abnormal bone healing / MGI
increased myocardial infarction size / MGI
allodynia / MGI
abnormal frequency of paradoxical sleep / MGI
neuron degeneration / MGI
liver fibrosis / MGI
decreased susceptibility to induced choroidal neovascularization / MGI
nervous system phenotype / MGI
abnormal physiological neovascularization / MGI
abnormal bone structure / MGI
increased left ventricle developed pressure / MGI
abnormal tooth hard tissue morphology / MGI
abnormal hormone level / MGI
abnormal adrenocorticotropin level / MGI
increased mortality induced by ionizing radiation / MGI
abnormal uterine artery morphology / MGI
decreased susceptibility to kidney reperfusion injury / MGI
abnormal peripheral nervous system regeneration / MGI
abnormal respiratory bronchiole morphology / MGI
increased length of allograft survival / MGI
abnormal surfactant physiology / MGI
increased susceptibility to experimental autoimmune encephalomyelitis / MGI
increased testis weight / MGI
increased mean systemic arterial blood pressure / MGI
decreased adiponectin level / MGI
skin inflammation / MGI
abnormal osteoclast morphology / MGI
decreased osteoclast cell number / MGI
abnormal brain wave pattern / MGI
decreased eosinophil cell number / MGI
abnormal response to infection / MGI
increased susceptibility to parasitic infection / MGI
increased circulating prolactin level / MGI
decreased susceptibility to injury / MGI
abnormal male meiosis / MGI
increased circulating cholesterol level / MGI
abnormal circulating estradiol level / MGI
improved glucose tolerance / MGI
abnormal heart ventricle morphology / MGI
abnormal response to tactile stimuli / MGI
abnormal enzyme/coenzyme level / MGI
abnormal myocardium layer morphology / MGI
atherosclerotic lesions / MGI
increased T cell proliferation / MGI
homeostasis/metabolism phenotype / MGI
cardiovascular system phenotype / MGI
immune system phenotype / MGI
respiratory system phenotype / MGI
reproductive system phenotype / MGI
abnormal fertilization / MGI
abnormal platelet physiology / MGI
abnormal T-helper 1 physiology / MGI
increased circulating creatinine level / MGI
increased renin activity / MGI
increased circulating antidiuretic hormone level / MGI
decreased circulating aspartate transaminase level / MGI
decreased circulating leptin level / MGI
decreased cerebral infarction size / MGI
increased systemic arterial systolic blood pressure / MGI
decreased systemic arterial systolic blood pressure / MGI
abnormal involution of the mammary gland / MGI
enlarged seminiferous tubules / MGI
increased single-positive T cell number / MGI
abnormal osteoclast differentiation / MGI
increased IgG2a level / MGI
decreased susceptibility to induced colitis / MGI
increased interferon-gamma secretion / MGI
decreased susceptibility to endotoxin shock / MGI
decreased survivor rate / MGI
prolonged diestrus / MGI
increased urine pH / MGI
abnormal platelet aggregation / MGI
enlarged inguinal lymph nodes / MGI
abnormal blood homeostasis / MGI
abnormal induced retinal neovascularization / MGI
increased sensitivity to induced morbidity/mortality / MGI
decreased oligodendrocyte number / MGI
abnormal heart right ventricle pressure / MGI
mortality/aging / MGI
increased bone volume / MGI
increased compact bone mass / MGI
decreased urine prostaglandin level / MGI
increased food intake / MGI
hyperlactation / MGI
abnormal circadian sleep/wake cycle / MGI
improved central nervous system regeneration / MGI
increased sperm number / MGI

Literature references

Mice lacking inducible nitric oxide synthase are not resistant to lipopolysaccharide-induced death.;Laubach V E, Shesely E G, Smithies O, Sherman P A, ;1995;Proceedings of the National Academy of Sciences of the United States of America;92;10688-92; 7479866

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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NOD.129P2(B6)-Nos2tm1Lau/Ieg