C57BL/6NTac-Arhgef1tm1a(EUCOMM)Wtsi/WtsiPh

Status

Available to order

EMMA IDEM:09891
International strain nameC57BL/6NTac-Arhgef1tm1a(EUCOMM)Wtsi/WtsiPh
Alternative nameEPD0177_5_G11
Strain typeTargeted Mutant Strains
Allele/Transgene symbolArhgef1tm1a(EUCOMM)Wtsi
Gene/Transgene symbolArhgef1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0177_5_G11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitute of Molecular Genetics, Prague, Czech Republic

Disease and phenotype information

IMPC phenotypes (allele matching)
  • increased monocyte cell number / IMPC
  • increased hematocrit / IMPC
  • increased erythrocyte cell number / IMPC
  • increased hemoglobin content / IMPC
  • increased CD4-positive, alpha-beta T cell number / IMPC
  • decreased CD8-positive, alpha-beta T cell number / IMPC
  • decreased dendritic cell number / IMPC
  • decreased marginal zone B cell number / IMPC
  • increased gamma-delta T cell number / IMPC
  • increased plasmacytoid dendritic cell number / IMPC
  • increased CD4-positive, alpha-beta memory T cell number / IMPC
  • increased CD8-positive, alpha-beta memory T cell number / IMPC
  • increased effector memory CD8-positive, alpha-beta T cell number / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
  • increased KLRG1-positive NK cell number / IMPC
  • increased Ly6C low monocyte number / IMPC
  • decreased memory-marker gamma-delta T cell number / IMPC
  • increased KLRG1-positive CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
  • decreased memory-marker CD4-positive NK T cell number / IMPC
  • decreased immature NK cell number / IMPC
  • decreased Ly6C-positive immature NK cell number / IMPC
  • increased mature NK cell number / IMPC
  • increased Ly6C-positive NK T cell number / IMPC
IMPC phenotypes (gene matching)
  • increased Ly6C-positive NK T cell number / IMPC
  • increased effector memory CD8-positive, alpha-beta T cell number / IMPC
  • increased CD4-positive, alpha-beta T cell number / IMPC
  • increased plasmacytoid dendritic cell number / IMPC
  • decreased immature NK cell number / IMPC
  • decreased CD8-positive, alpha-beta T cell number / IMPC
  • decreased memory-marker CD4-positive NK T cell number / IMPC
  • increased KLRG1-positive NK cell number / IMPC
  • increased hematocrit / IMPC
  • decreased marginal zone B cell number / IMPC
  • increased hemoglobin content / IMPC
  • increased KLRG1-positive CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
  • increased gamma-delta T cell number / IMPC
  • decreased memory-marker gamma-delta T cell number / IMPC
  • increased erythrocyte cell number / IMPC
  • increased Ly6C low monocyte number / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
  • increased CD4-positive, alpha-beta memory T cell number / IMPC
  • decreased Ly6C-positive immature NK cell number / IMPC
  • decreased dendritic cell number / IMPC
  • increased CD8-positive, alpha-beta memory T cell number / IMPC
  • increased monocyte cell number / IMPC
  • increased mature NK cell number / IMPC
MGI phenotypes (gene matching)
  • increased leukocyte cell number / MGI
  • extramedullary hematopoiesis / MGI
  • enlarged spleen / MGI
  • abnormal lymphocyte cell number / MGI
  • abnormal humoral immune response / MGI
  • decreased IgG level / MGI
  • decreased IgM level / MGI
  • abnormal neutrophil physiology / MGI
  • abnormal lymphocyte physiology / MGI
  • decreased B-1 B cell number / MGI
  • decreased B cell number / MGI
  • decreased B cell proliferation / MGI
  • increased T cell proliferation / MGI
  • decreased follicular B cell number / MGI
  • increased germinal center B cell number / MGI
  • decreased marginal zone B cell number / MGI
  • abnormal marginal zone B cell physiology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).