C57BL/6NTac-Arhgef1^{tm1a(EUCOMM)Wtsi}/WtsiPh

Status	Available to order
EMMA ID	EM:09891
International strain name	C57BL/6NTac-Arhgef1^{tm1a(EUCOMM)Wtsi}/WtsiPh
Alternative name	EPD0177_5_G11
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Arhgef1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Arhgef1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0177_5_G11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institute of Molecular Genetics, Prague, Czech Republic

Disease and phenotype information

IMPC phenotypes (allele matching)

increased monocyte cell number / IMPC
increased hematocrit / IMPC
increased erythrocyte cell number / IMPC
increased hemoglobin content / IMPC
increased CD4-positive, alpha-beta T cell number / IMPC
decreased CD8-positive, alpha-beta T cell number / IMPC
decreased dendritic cell number / IMPC
decreased marginal zone B cell number / IMPC
increased gamma-delta T cell number / IMPC
increased plasmacytoid dendritic cell number / IMPC
increased CD4-positive, alpha-beta memory T cell number / IMPC
increased CD8-positive, alpha-beta memory T cell number / IMPC
increased effector memory CD8-positive, alpha-beta T cell number / IMPC
preweaning lethality, incomplete penetrance / IMPC
increased KLRG1-positive NK cell number / IMPC
increased Ly6C low monocyte number / IMPC
decreased memory-marker gamma-delta T cell number / IMPC
increased KLRG1-positive CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
decreased memory-marker CD4-positive NK T cell number / IMPC
decreased immature NK cell number / IMPC
decreased Ly6C-positive immature NK cell number / IMPC
increased mature NK cell number / IMPC
increased Ly6C-positive NK T cell number / IMPC

IMPC phenotypes (gene matching)

increased Ly6C-positive NK T cell number / IMPC
increased effector memory CD8-positive, alpha-beta T cell number / IMPC
increased CD4-positive, alpha-beta T cell number / IMPC
increased plasmacytoid dendritic cell number / IMPC
decreased immature NK cell number / IMPC
decreased CD8-positive, alpha-beta T cell number / IMPC
decreased memory-marker CD4-positive NK T cell number / IMPC
increased KLRG1-positive NK cell number / IMPC
increased hematocrit / IMPC
decreased marginal zone B cell number / IMPC
increased hemoglobin content / IMPC
increased KLRG1-positive CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
increased gamma-delta T cell number / IMPC
decreased memory-marker gamma-delta T cell number / IMPC
increased erythrocyte cell number / IMPC
increased Ly6C low monocyte number / IMPC
preweaning lethality, incomplete penetrance / IMPC
increased CD4-positive, alpha-beta memory T cell number / IMPC
decreased Ly6C-positive immature NK cell number / IMPC
decreased dendritic cell number / IMPC
increased CD8-positive, alpha-beta memory T cell number / IMPC
increased monocyte cell number / IMPC
increased mature NK cell number / IMPC

MGI phenotypes (gene matching)

increased leukocyte cell number / MGI
extramedullary hematopoiesis / MGI
enlarged spleen / MGI
abnormal lymphocyte cell number / MGI
abnormal humoral immune response / MGI
decreased IgG level / MGI
decreased IgM level / MGI
abnormal neutrophil physiology / MGI
abnormal lymphocyte physiology / MGI
decreased B-1 B cell number / MGI
decreased B cell number / MGI
decreased B cell proliferation / MGI
increased T cell proliferation / MGI
decreased follicular B cell number / MGI
increased germinal center B cell number / MGI
decreased marginal zone B cell number / MGI
abnormal marginal zone B cell physiology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Arhgef1tm1a(EUCOMM)Wtsi/WtsiPh