C57BL/6N-A^tm1Brd Tet1^{tm1a(KOMP)Wtsi}/WtsiOrl

Status	Available to order
EMMA ID	EM:09888
International strain name	C57BL/6N-A^tm1Brd Tet1^{tm1a(KOMP)Wtsi}/WtsiOrl
Alternative name	EPD0850_2_B07
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Tet1^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Tet1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0850_2_B07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6NTac

Disease and phenotype information

MGI phenotypes (gene matching)

midline facial cleft / MGI
decreased neutrophil cell number / MGI
irregular coat pigmentation / MGI
abnormal lateral ventricle morphology / MGI
incomplete rostral neuropore closure / MGI
small ovary / MGI
decreased body length / MGI
decreased body weight / MGI
decreased body size / MGI
ocular hypertelorism / MGI
abnormal temporal memory / MGI
abnormal embryo development / MGI
anencephaly / MGI
decreased litter size / MGI
abnormal forebrain development / MGI
absent ovary / MGI
nervous system phenotype / MGI
abnormal female reproductive system physiology / MGI
abnormal neural tube closure / MGI
embryonic growth retardation / MGI
decreased ovary weight / MGI
abnormal female meiosis / MGI
reproductive system phenotype / MGI
decreased oocyte number / MGI
abnormal interfrontal bone morphology / MGI
abnormal paraventricular hypothalamic nucleus morphology / MGI
abnormal supraoptic nucleus morphology / MGI
abnormal synaptonemal complex / MGI
decreased birth body size / MGI
increased lipoma incidence / MGI
abnormal double-strand DNA break repair / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
preweaning lethality, complete penetrance / MGI
prenatal lethality, incomplete penetrance / MGI
excessive folding of visceral yolk sac / MGI
abnormal head fold morphology / MGI
encephalomeningocele / MGI
impaired spatial learning / MGI
abnormal lamina terminalis morphology / MGI
bifid nose / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

Search

INFRAFRONTIER^® and European Mouse Mutant Archive - EMMA^® are registered trademarks at the European Union Intellectual Property Office (EUIPO).