MGI phenotypes (gene matching)
B6.Cg-Piwil4tm1Embrp/Cnrm
| Status | Available to order |
| EMMA ID | EM:09867 |
| Citation information | RRID:IMSR_EM:09867 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Piwil4tm1Embrp/Cnrm |
| Alternative name | NHA-Piwil4 knock-in mouse |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Piwil4tm1Embrp |
| Gene/Transgene symbol | Piwil4 |
Information from provider
| Provider | Ramesh PILLAI |
| Provider affiliation | Mouse Biology Unit, European Molecular Biology Laboratory |
| Genetic information | Piwil4 (common name: Miwi2) protein with a N-HA tag knocked-in before the ATG. The N-peptide tag is suitable for artificial tethering of the protein to a reporter with BoxB hairpins. 1X HA tag allows detection of the protein. The mouse is to be used for artificial tethering of Piwi4 to a reporter, to study role of Piwil4 in transcriptional repression via recruitment of DNA methylation. |
| Phenotypic information | Homozygous:No observable impact on viability or fertility. The mouse is a valuable tool to study small RNA-guided DNA methylation in mammals.Heterozygous:No observable impact on viability or fertility. The mouse is a valuable tool to study small RNA-guided DNA methylation in mammals. |
| Breeding history | Founder animals (generated from modified A9 cells) were crossed with siblings or with wild-type C57BL/6 mice. No observable effect on viability or fertility. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |