B6.Cg-Piwil4tm1Embrp/Cnrm
Status | Available to order |
EMMA ID | EM:09867 |
International strain name | B6.Cg-Piwil4tm1Embrp/Cnrm |
Alternative name | NHA-Piwil4 knock-in mouse |
Strain type | Targeted Mutant Strains : Knock-in |
Allele/Transgene symbol | Piwil4tm1Embrp |
Gene/Transgene symbol | Piwil4 |
Information from provider
Provider | Ramesh PILLAI |
Provider affiliation | Mouse Biology Unit, European Molecular Biology Laboratory |
Genetic information | Piwil4 (common name: Miwi2) protein with a N-HA tag knocked-in before the ATG. The N-peptide tag is suitable for artificial tethering of the protein to a reporter with BoxB hairpins. 1X HA tag allows detection of the protein. The mouse is to be used for artificial tethering of Piwi4 to a reporter, to study role of Piwil4 in transcriptional repression via recruitment of DNA methylation. |
Phenotypic information | Homozygous:No observable impact on viability or fertility. The mouse is a valuable tool to study small RNA-guided DNA methylation in mammals.Heterozygous:No observable impact on viability or fertility. The mouse is a valuable tool to study small RNA-guided DNA methylation in mammals. |
Breeding history | Founder animals (generated from modified A9 cells) were crossed with siblings or with wild-type C57BL/6 mice. No observable effect on viability or fertility. |
References | None available |
Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
MGI phenotypes (gene matching)
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