B6.129S2-Parp1^tm1Jmdm/Biat

Status	Available to order
EMMA ID	EM:09855
International strain name	B6.129S2-Parp1^tm1Jmdm/Biat
Alternative name	PARP1
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Parp1^tm1Jmdm
Gene/Transgene symbol	Parp1

Information from provider

Provider	Gilbert de Murcia
Provider affiliation	UMR7242, CNRS
Additional owner	Francoise Dantzer and Valérie Schreiber, CNRS-UMR7242, Illkirch, France
Genetic information	The Parp1 gene has been inactivated by homologous recombination in ES cells from the 129/Sv mouse line by inserting the PGK-neo in exon 4.
Phenotypic information	Homozygous: The mice display sensitivity to DNA damaging agents (IR, MNU). The mice are viable and fertile. Heterozygous: No phenotype for the heterozygotes.
References	Requirement of poly(ADP-ribose) polymerase in recovery from DNA damage in mice and in cells.;de Murcia J M, Niedergang C, Trucco C, Ricoul M, Dutrillaux B, Mark M, Oliver F J, Masson M, Dierich A, LeMeur M, Walztinger C, Chambon P, de Murcia G, ;1997;Proceedings of the National Academy of Sciences of the United States of America;94;7303-7; 9207086
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	University of Veterinary Medicine, Vienna, Austria
Breeding at archiving centre	Parp1 (EMMA ID EM:09855) and Parp2 (EMMA ID EM:09858) strains have been imported as refrig. epid.; SF males were produced out of these, sperm frozen and quality-controlled. Therefore prolonged archiving period.

Disease and phenotype information

IMPC phenotypes (gene matching)

decreased grip strength / IMPC
decreased total retina thickness / IMPC
decreased NK cell number / IMPC
preweaning lethality, incomplete penetrance / IMPC
abnormal spleen morphology / IMPC
abnormal uterus morphology / IMPC
abnormal eye morphology / IMPC
increased prepulse inhibition / IMPC
small seminal vesicle / IMPC
immune system phenotype / IMPC
increased circulating creatinine level / IMPC
increased fasting circulating glucose level / IMPC
abnormal seminal vesicle morphology / IMPC
microphthalmia / IMPC
increased effector memory CD8-positive, alpha-beta T cell number / IMPC
enlarged spleen / IMPC
abnormal retina inner nuclear layer morphology / IMPC

MGI phenotypes (allele matching)

increased mortality induced by ionizing radiation / MGI
immune system phenotype / MGI
decreased body weight / MGI
increased mortality induced by gamma-irradiation / MGI
abnormal inflammatory response / MGI
decreased litter size / MGI
abnormal leukocyte migration / MGI
elevated level of mitotic sister chromatid exchange / MGI
induced chromosome breakage / MGI
abnormal cell cycle checkpoint function / MGI
decreased susceptibility to noise-induced hearing loss / MGI
increased apoptosis / MGI
abnormal DNA repair / MGI
increased sensitivity to induced morbidity/mortality / MGI
prenatal lethality, incomplete penetrance / MGI

MGI phenotypes (gene matching)

decreased cell proliferation / MGI
skin hyperplasia / MGI
decreased body weight / MGI
increased mortality induced by gamma-irradiation / MGI
abnormal inflammatory response / MGI
decreased inflammatory response / MGI
decreased litter size / MGI
abnormal macrophage physiology / MGI
enhanced wound healing / MGI
no phenotypic analysis / MGI
abnormal leukocyte migration / MGI
decreased neuron apoptosis / MGI
abnormal response/metabolism to endogenous compounds / MGI
abnormal microglial cell physiology / MGI
elevated level of mitotic sister chromatid exchange / MGI
increased mortality induced by ionizing radiation / MGI
induced chromosome breakage / MGI
decreased susceptibility to kidney reperfusion injury / MGI
abnormal cell cycle checkpoint function / MGI
decreased susceptibility to noise-induced hearing loss / MGI
decreased susceptibility to injury / MGI
immune system phenotype / MGI
increased renal glomerular filtration rate / MGI
decreased circulating creatinine level / MGI
increased apoptosis / MGI
abnormal DNA repair / MGI
decreased circulating tumor necrosis factor level / MGI
decreased circulating interleukin-1 beta level / MGI
decreased susceptibility to endotoxin shock / MGI
decreased physiological sensitivity to xenobiotic / MGI
increased sensitivity to induced morbidity/mortality / MGI
prenatal lethality, incomplete penetrance / MGI
decreased renal tubule apoptosis / MGI
decreased susceptibility to dopaminergic neuron neurotoxicity / MGI

Literature references

Requirement of poly(ADP-ribose) polymerase in recovery from DNA damage in mice and in cells.;de Murcia J M, Niedergang C, Trucco C, Ricoul M, Dutrillaux B, Mark M, Oliver F J, Masson M, Dierich A, LeMeur M, Walztinger C, Chambon P, de Murcia G, ;1997;Proceedings of the National Academy of Sciences of the United States of America;94;7303-7; 9207086

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6.129S2-Parp1tm1Jmdm/Biat