C57BL/6NTac-Bhlhe40tm1a(KOMP)Wtsi/WtsiPh

Status

Available to order

EMMA IDEM:09819
Citation informationRRID:IMSR_EM:09819 

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International strain nameC57BL/6NTac-Bhlhe40tm1a(KOMP)Wtsi/WtsiPh
Alternative nameEPD0208_6_A02
Strain typeTargeted Mutant Strains
Allele/Transgene symbolBhlhe40tm1a(KOMP)Wtsi
Gene/Transgene symbolBhlhe40
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from KOMP ES clone EPD0208_6_A02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitute of Molecular Genetics, Prague, Czech Republic

Disease and phenotype information

IMPC phenotypes (gene matching)
  • increased heart weight / IMPC
  • increased blood urea nitrogen level / IMPC
  • increased circulating fructosamine level / IMPC
  • increased mean corpuscular hemoglobin / IMPC
  • decreased circulating calcium level / IMPC
  • decreased circulating HDL cholesterol level / IMPC
  • increased circulating chloride level / IMPC
  • decreased fasting circulating glucose level / IMPC
  • increased circulating iron level / IMPC
  • decreased circulating glycerol level / IMPC
  • decreased circulating cholesterol level / IMPC
MGI phenotypes (gene matching)
  • spleen hyperplasia / MGI
  • thymus hyperplasia / MGI
  • abnormal immune system cell morphology / MGI
  • abnormal immune system physiology / MGI
  • abnormal T cell activation / MGI
  • altered susceptibility to autoimmune disorder / MGI
  • increased IgE level / MGI
  • increased susceptibility to pharmacologically induced seizures / MGI
  • no phenotypic analysis / MGI
  • increased anti-nuclear antigen antibody level / MGI
  • increased double-negative T cell number / MGI
  • decreased double-positive T cell number / MGI
  • decreased T cell proliferation / MGI
  • abnormal renal glomerulus morphology / MGI
  • abnormal CD4-positive, alpha-beta T cell physiology / MGI
  • increased CD4-positive, alpha beta T cell number / MGI
  • abnormal CD4-positive T cell differentiation / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • lymph node hyperplasia / MGI
  • increased spleen white pulp amount / MGI
  • increased spleen germinal center number / MGI
  • increased IgG1 level / MGI
  • abnormal lymph node germinal center morphology / MGI
  • decreased interleukin-2 secretion / MGI
  • delayed circadian behavior phase / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

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More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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