StarD10-F10
| Status | Available to order |
| EMMA ID | EM:09784 |
| International strain name | StarD10-F10 |
| Alternative name | StarD10-F10 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Unknown at present |
| Gene/Transgene symbol | mouse StarD10 |
Information from provider
| Provider | Guy Rutter |
| Provider affiliation | Medicine, Imperial College London |
| Genetic information | Insertion of a coding cassette under the control of a bidirectional tetracycline-regulated promoter driving expression of both mouse Flag-StarD10 and firefly luciferase. Vector used for the subcloning of the transgene incorporated on this strain: pBI-L Vector PT3069-5 GenBank Accession No.:U89934 Catalog No. 631005 |
| Phenotypic information | Homozygous:N/AHeterozygous:Overexpression of the transgene when crossed with mouse expressing the reverse tetracycline transactivator and given doxycycline (possibility of a tissue specific overexpression). The transgene copy number for this line was evaluated by qPCR and estimated at 25 copies ([23.88 - 25.43], n = 14). The expression level in islets was measured by Western blot after induction by 2 g/L doxycycline in drinking water and estimated to be 120% of the control expression. In vivo phenotype: The animals were crossed with RIP7-rtTA mice allowing overexpression of the transgene specifically in pancreatic beta-cells. The doxycycline was added in drinking water (2 g/L) from 5 weeks of age and intra-peritoneal glucose tolerance tests (IPGTT, 1 g/kg glucose) were performed at 8, 12 and 16-week-old. The resulting line had no differences with control mice on chow diet. This line has not yet been tested on HFD. |
| Breeding history | Founder crossed with wild-type C57BL/6J to generate F1; F1 crossed with wild-type C57BL/6J to generate F2 to ensure the stability of the line (one site of insertion only). |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |