C57BL/6-Tg(tetO-luc,-Acot7)F26Rttr/H

Status

Available to order

EMMA IDEM:09783
International strain nameC57BL/6-Tg(tetO-luc,-Acot7)F26Rttr/H
Alternative nameAcot7 tg
Strain typeTransgenic Strains
Allele/Transgene symbolTg(tetO-luc,-Acot7)F26Rttr
Gene/Transgene symbolTg(tetO-luc,-Acot7)F26Rttr

Information from provider

ProviderGuy Rutter
Provider affiliationSection of Cell Biology, Department of Medicine, Imperial College London
Genetic informationMice express Acot7 cDNA under the control of a tetracycline-inducible bidirectional promoter. Firefly luciferase is expressed simultaneously under the control of the same promoter. Insertion was via pronuclear injection into C57BL/6J pronuclei; integration was aleatory - we do not know the location in the genome. Vector used for the subcloning of the transgene incorporated on this strain: pBI-L Vector PT3069-5 GenBank Accession No.:U89934 Catalog No. 631005
Phenotypic informationHomozygous:
We have not studied homozygous mice. No phenotype should be observed in the absence of further breeding with animals expressing rtTA and in the presence of doxycycline.

Heterozygous:
When bred with animals expressing rtTA specifically in pancreatic beta cells, and upon doxycycline administration, the mice develop mild glucose intolerance, impaired insulin secretion in response to glucose and the islets present reduced levels of fatty acid-CoA.
Breeding historyThe founder animal was first bred with C57BL/6 wild-type, and the F1 again with C57BL/6 wild-type to confirm only one insertion by qPCR. F2 was bred with littermate controls (not containing the transgene) or C57BL/6 wild-type for at least 4 generations.
References
  • Disallowance of Acot7 in β-Cells Is Required for Normal Glucose Tolerance and Insulin Secretion.;Martinez-Sanchez Aida, Pullen Timothy J, Chabosseau Pauline, Zhang Qifeng, Haythorne Elizabeth, Cane Matthew C, Nguyen-Tu Marie-Sophie, Sayers Sophie R, Rutter Guy A, ;2016;Diabetes;65;1268-82; 26861785
Homozygous fertilenot known
Homozygous viableno
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Literature references

  • Disallowance of Acot7 in β-Cells Is Required for Normal Glucose Tolerance and Insulin Secretion.;Martinez-Sanchez Aida, Pullen Timothy J, Chabosseau Pauline, Zhang Qifeng, Haythorne Elizabeth, Cane Matthew C, Nguyen-Tu Marie-Sophie, Sayers Sophie R, Rutter Guy A, ;2016;Diabetes;65;1268-82; 26861785

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

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