C57BL/6N-Pla2g6^{tm1a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:09779
International strain name	C57BL/6N-Pla2g6^{tm1a(EUCOMM)Wtsi}/WtsiH
Alternative name	Pla2g6-G04
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Pla2g6^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Pla2g6
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone Pla2g6-G04. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Infantile neuroaxonal dystrophy / Orphanet_35069
Adult-onset dystonia-parkinsonism / Orphanet_199351

IMPC phenotypes (allele matching)

male infertility / IMPC

IMPC phenotypes (gene matching)

male infertility / IMPC

MGI phenotypes (gene matching)

kyphosis / MGI
tremors / MGI
hindlimb paralysis / MGI
abnormal spinal cord morphology / MGI
weight loss / MGI
ataxia / MGI
hyperactivity / MGI
impaired coordination / MGI
abnormal gait / MGI
increased exploration in new environment / MGI
abnormal motor coordination/balance / MGI
impaired limb coordination / MGI
reduced male fertility / MGI
male infertility / MGI
blindness / MGI
abnormal motor capabilities/coordination/movement / MGI
premature death / MGI
abnormal brain morphology / MGI
neurodegeneration / MGI
muscular atrophy / MGI
abnormal sciatic nerve morphology / MGI
asthenozoospermia / MGI
axonal dystrophy / MGI
abnormal locomotor coordination / MGI
abnormal nervous system morphology / MGI
abnormal myelin sheath morphology / MGI
increased cholesterol level / MGI
decreased double-positive T cell number / MGI
abnormal spinal cord ventral horn morphology / MGI
abnormal brainstem morphology / MGI
abnormal axon morphology / MGI
axon degeneration / MGI
abnormal mitochondrion morphology / MGI
abnormal brain white matter morphology / MGI
abnormal spinal cord grey matter morphology / MGI
abnormal synaptic bouton morphology / MGI
abnormal astrocyte physiology / MGI
skeletal muscle atrophy / MGI
axonal spheroids / MGI
decreased grip strength / MGI
abnormal apolipoprotein level / MGI
abnormal mitochondrial crista morphology / MGI
abnormal cell cytoskeleton morphology / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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