C57BL/6NTac-Klk5tm1(EGFP/cre/ERT2)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:09760
International strain nameC57BL/6NTac-Klk5tm1(EGFP/cre/ERT2)Wtsi/WtsiH
Alternative nameCEPD0049_3_D09
Strain type
Allele/Transgene symbolKlk5tm1(EGFP/cre/ERT2)Wtsi
Gene/Transgene symbolKlk5

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone CEPD0049_3_D09. For further details on the construction of this clone see the page at the IMPC portal.
Quality Control/Disclaimer: The structure of the targeted mutation is not fully verified. It is recommended that the recipient confirms the mutation structure by Southern blotting. The Cre expression pattern is expected to mirror that of the host gene, but this may not be fully described and/or may be modified in the targeted allele. Although characterisation of the Cre expression pattern is an objective of EUCOMMTools this is an ongoing process which may be incomplete. It is therefore recommended that the recipient verifies the Cre expression pattern. For the tm1(EGFP/Cre/ERT2) type allele, if the promoter-driven selection cassette is flanked with Rox sites, it may be removed using Dre recombinase. Please be aware that the strain description may be subject to change. For the most recent description please consult the information pages for the appropriate product details at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)
  • abnormal auditory brainstem response / IMPC
  • decreased mean corpuscular hemoglobin concentration / IMPC
  • increased mean corpuscular volume / IMPC
  • abnormal retina blood vessel morphology / IMPC
  • abnormal vitreous body morphology / IMPC
  • decreased total retina thickness / IMPC
  • decreased locomotor activity / IMPC
  • increased red blood cell distribution width / IMPC
  • abnormal retina vasculature morphology / IMPC
  • long tibia / IMPC
  • decreased startle reflex / IMPC
  • abnormal retina morphology / IMPC
  • increased circulating phosphate level / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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