C57BL/6NTac-Phextm1(EGFP/cre/ERT2)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:09732
International strain nameC57BL/6NTac-Phextm1(EGFP/cre/ERT2)Wtsi/WtsiH
Alternative nameCEPD0045_5_C09
Strain type
Allele/Transgene symbolPhextm1(EGFP/cre/ERT2)Wtsi
Gene/Transgene symbolPhex

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone CEPD0045_5_C09. For further details on the construction of this clone see the page at the IMPC portal.
Quality Control/Disclaimer: The structure of the targeted mutation is not fully verified. It is recommended that the recipient confirms the mutation structure by Southern blotting. The Cre expression pattern is expected to mirror that of the host gene, but this may not be fully described and/or may be modified in the targeted allele. Although characterisation of the Cre expression pattern is an objective of EUCOMMTools this is an ongoing process which may be incomplete. It is therefore recommended that the recipient verifies the Cre expression pattern. For the tm1(EGFP/Cre/ERT2) type allele, if the promoter-driven selection cassette is flanked with Rox sites, it may be removed using Dre recombinase. Please be aware that the strain description may be subject to change. For the most recent description please consult the information pages for the appropriate product details at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • abnormal vertebral arch morphology / IMPC
  • short tail / IMPC
  • decreased blood urea nitrogen level / IMPC
  • decreased heart weight / IMPC
  • decreased body length / IMPC
  • abnormal vertebrae morphology / IMPC
  • decreased circulating calcium level / IMPC
  • thin tail / IMPC
  • abnormal body size / IMPC
  • abnormal radius morphology / IMPC
  • abnormal ulna morphology / IMPC
  • abnormal bone mineralization / IMPC
  • decreased circulating phosphate level / IMPC
  • decreased circulating amylase level / IMPC
  • decreased body weight / IMPC
  • decreased circulating triglyceride level / IMPC
  • increased circulating potassium level / IMPC
  • abnormal joint morphology / IMPC
  • decreased bone mineral density / IMPC
  • abnormal tail movements / IMPC
  • abnormal tibia morphology / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • increased bone mineral content / IMPC
  • abnormal tail morphology / IMPC
MGI phenotypes (gene matching)
  • decreased bone mineral density / MGI
  • short mandible / MGI
  • short premaxilla / MGI
  • abnormal long bone metaphysis morphology / MGI
  • decreased compact bone thickness / MGI
  • abnormal vertebrae morphology / MGI
  • abnormal rib morphology / MGI
  • decreased circulating calcium level / MGI
  • decreased circulating phosphate level / MGI
  • abnormal cranium morphology / MGI
  • domed cranium / MGI
  • short limbs / MGI
  • abnormal hindlimb morphology / MGI
  • short tail / MGI
  • decreased body length / MGI
  • decreased body weight / MGI
  • decreased body size / MGI
  • circling / MGI
  • abnormal gait / MGI
  • abnormal osteoclast physiology / MGI
  • abnormal intestinal absorption / MGI
  • postnatal growth retardation / MGI
  • abnormal postnatal growth/weight/body size / MGI
  • disproportionate dwarf / MGI
  • abnormal caudal vertebrae morphology / MGI
  • short tibia / MGI
  • short fibula / MGI
  • abnormal cranial suture morphology / MGI
  • abnormal bone mineralization / MGI
  • increased urine phosphate level / MGI
  • increased circulating parathyroid hormone level / MGI
  • abnormal joint morphology / MGI
  • decreased circulating alkaline phosphatase level / MGI
  • increased circulating alkaline phosphatase level / MGI
  • decreased urine calcium level / MGI
  • abnormal long bone epiphyseal plate morphology / MGI
  • short femur / MGI
  • rickets / MGI
  • increased width of hypertrophic chondrocyte zone / MGI
  • increased activity of parathyroid / MGI
  • maternal effect / MGI
  • abnormal long bone morphology / MGI
  • abnormal bone structure / MGI
  • small clavicle / MGI
  • short humerus / MGI
  • short radius / MGI
  • short ulna / MGI
  • short frontal bone / MGI
  • short nasal bone / MGI
  • abnormal pelvic girdle bone morphology / MGI
  • small vertebrae / MGI
  • small caudal vertebrae / MGI
  • decreased length of long bones / MGI
  • decreased bone resorption / MGI
  • renal/urinary system phenotype / MGI
  • cellular phenotype / MGI
  • cardiovascular system phenotype / MGI
  • abnormal skeleton morphology / MGI
  • increased diameter of femur / MGI
  • decreased cranium height / MGI
  • osteomalacia / MGI
  • abnormal blood homeostasis / MGI
  • exostosis / MGI
  • decreased intestinal calcium absorption / MGI
  • abnormal limb long bone morphology / MGI
  • camptomelia / MGI
  • abnormal obturator foramen morphology / MGI
  • abnormal acetabulum morphology / MGI
  • abnormal costochondral joint morphology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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