C57BL/6N-Atm1Brd Irf5tm1e(EUCOMM)Wtsi/WtsiH

Status

Available to order

EMMA IDEM:09730
International strain nameC57BL/6N-Atm1Brd Irf5tm1e(EUCOMM)Wtsi/WtsiH
Alternative nameEPD0594_2_A02
Strain typeTargeted Mutant Strains : Targeted Non-conditional
Allele/Transgene symbolIrf5tm1e(EUCOMM)Wtsi
Gene/Transgene symbolIrf5
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0594_2_A02. For further details on the construction of this clone see the page at the IMPC portal. The targeted allele has lost the 3' loxP site. These mutations cannot be converted into conditional alleles. Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)
  • decreased leukocyte cell number / IMPC
  • decreased CD4-positive, alpha-beta memory T cell number / IMPC
IMPC phenotypes (gene matching)
  • preweaning lethality, complete penetrance / IMPC
  • decreased CD4-positive, alpha-beta memory T cell number / IMPC
  • decreased leukocyte cell number / IMPC
MGI phenotypes (gene matching)
  • no abnormal phenotype detected / MGI
  • decreased susceptibility to bacterial infection / MGI
  • abnormal response to infection / MGI
  • immune system phenotype / MGI
  • abnormal T-helper 1 physiology / MGI
  • decreased circulating tumor necrosis factor level / MGI
  • decreased tumor necrosis factor secretion / MGI
  • decreased interferon-alpha secretion / MGI
  • decreased circulating interleukin-6 level / MGI
  • decreased circulating interleukin-12b level / MGI
  • decreased interleukin-6 secretion / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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