B6;129-Vegfatm2Gne Tg(SFTPC-rtTA)5Jaw Tg(tetO-cre)1Jaw/H
| Status | Available to order |
| EMMA ID | EM:09724 |
| Citation information | RRID:IMSR_EM:09724 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129-Vegfatm2Gne Tg(SFTPC-rtTA)5Jaw Tg(tetO-cre)1Jaw/H |
| Alternative name | C57/BL6 - SPC tetCre loxP VEGF |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(SFTPC-rtTA)5Jaw, Vegfatm2Gne, Tg(tetO-cre)1Jaw |
| Gene/Transgene symbol | Tg(SFTPC-rtTA)5Jaw , Vegfa, Tg(tetO-cre)1Jaw |
Information from provider
| Provider | Ann Millar |
| Provider affiliation | Clinical Sciences - Academic Respiratory Unit, University of Bristol |
| Additional owner | Dr. Shaney Barratt, University of Bristol, Bristol, UK |
| Genetic information | tetO-cre transgene and Vegfa loxP-flanked conditional mutation under control of a SFTPC-rtTA (Tg-SPC-rtTA) transgene with human surfactant protein c promoter linked to reverse tetracycline responsive transactivator. Also called "Tet-On" system with Tet response element (pTRE or PTre) promoter. |
| Phenotypic information | Homozygous:unknownHeterozygous:Transgenic males and females appear healthy. Doxycycline is required to induce transgene expression. Again no overt phenotypic presentation seen in mice to at least 30 weeks old. |
| Breeding history | Sib-mated for more than 10 generations. Crosses of Tg-tetOCre (+/-) loxP-Vegfa (+/+) with Tg-SPC-rtTA (+/-) loxP-Vegfa (+/+) mice or by crossing Tg-SPC-rtTA (+/-) Tg-tetOCre (+/-) loxP-Vegfa (+/+) mice with loxP-Vegfa (+/+) mice. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | heterozygous C57BL/6 males |
| Breeding at archiving centre | Cross to C57BL/6 |
Literature references
- Differential Expression of VEGF-Axxx Isoforms Is Critical for Development of Pulmonary Fibrosis.;Barratt Shaney L, Blythe Thomas, Jarrett Caroline, Ourradi Khadija, Shelley-Fraser Golda, Day Michael J, Qiu Yan, Harper Steve, Maher Toby M, Oltean Sebastian, Hames Thomas J, Scotton Chris J, Welsh Gavin I, Bates David O, Millar Ann B, ;2017;American journal of respiratory and critical care medicine;196;479-493; 28661183