B6;129-Vegfa^tm2Gne Tg(SFTPC-rtTA)5Jaw Tg(tetO-cre)1Jaw/H

Status	Available to order
EMMA ID	EM:09724
International strain name	B6;129-Vegfa^tm2Gne Tg(SFTPC-rtTA)5Jaw Tg(tetO-cre)1Jaw/H
Alternative name	C57/BL6 - SPC tetCre loxP VEGF
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(SFTPC-rtTA)5Jaw, Vegfa^tm2Gne, Tg(tetO-cre)1Jaw
Gene/Transgene symbol	Tg(SFTPC-rtTA)5Jaw , Vegfa, Tg(tetO-cre)1Jaw

Information from provider

Provider	Ann Millar
Provider affiliation	Clinical Sciences - Academic Respiratory Unit, University of Bristol
Additional owner	Dr. Shaney Barratt, University of Bristol, Bristol, UK
Genetic information	tetO-cre transgene and Vegfa loxP-flanked conditional mutation under control of a SFTPC-rtTA (Tg-SPC-rtTA) transgene with human surfactant protein c promoter linked to reverse tetracycline responsive transactivator. Also called "Tet-On" system with Tet response element (pTRE or PTre) promoter.
Phenotypic information	Homozygous: unknown Heterozygous: Transgenic males and females appear healthy. Doxycycline is required to induce transgene expression. Again no overt phenotypic presentation seen in mice to at least 30 weeks old.
Breeding history	Sib-mated for more than 10 generations. Crosses of Tg-tetOCre (+/-) loxP-Vegfa (+/+) with Tg-SPC-rtTA (+/-) loxP-Vegfa (+/+) mice or by crossing Tg-SPC-rtTA (+/-) Tg-tetOCre (+/-) loxP-Vegfa (+/+) mice with loxP-Vegfa (+/+) mice.
References	Differential Expression of VEGF-A_xxx Isoforms Is Critical for Development of Pulmonary Fibrosis.;Barratt Shaney L, Blythe Thomas, Jarrett Caroline, Ourradi Khadija, Shelley-Fraser Golda, Day Michael J, Qiu Yan, Harper Steve, Maher Toby M, Oltean Sebastian, Hames Thomas J, Scotton Chris J, Welsh Gavin I, Bates David O, Millar Ann B, ;2017;American journal of respiratory and critical care medicine;196;479-493; 28661183
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	yes
Immunocompromised	no

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6
Breeding at archiving centre	Cross to C57BL/6

Literature references

Differential Expression of VEGF-A_xxx Isoforms Is Critical for Development of Pulmonary Fibrosis.;Barratt Shaney L, Blythe Thomas, Jarrett Caroline, Ourradi Khadija, Shelley-Fraser Golda, Day Michael J, Qiu Yan, Harper Steve, Maher Toby M, Oltean Sebastian, Hames Thomas J, Scotton Chris J, Welsh Gavin I, Bates David O, Millar Ann B, ;2017;American journal of respiratory and critical care medicine;196;479-493; 28661183

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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B6;129-Vegfatm2Gne Tg(SFTPC-rtTA)5Jaw Tg(tetO-cre)1Jaw/H