C57BL/6NTac-Dclk1^{tm1a(EUCOMM)Wtsi}/WtsiOrl

Status	Available to order
EMMA ID	EM:09711
International strain name	C57BL/6NTac-Dclk1^{tm1a(EUCOMM)Wtsi}/WtsiOrl
Alternative name	MEPD1000_3_H02
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Dclk1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Dclk1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone MEPD1000_3_H02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Functional analysis of candidate genes from genome-wide association studies of hearing.;Ingham Neil J, Rook Victoria, Di Domenico Francesca, James Elysia, Lewis Morag A, Girotto Giorgia, Buniello Annalisa, Steel Karen P, ;2020;Hearing research;387;107879; 31927188

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6NTac

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased body length / IMPC
increased anti-nuclear antigen antibody level / IMPC
increased circulating aspartate transaminase level / IMPC
increased grip strength / IMPC
decreased circulating magnesium level / IMPC

IMPC phenotypes (gene matching)

increased anti-nuclear antigen antibody level / IMPC
increased grip strength / IMPC
decreased circulating magnesium level / IMPC
increased circulating aspartate transaminase level / IMPC
decreased body length / IMPC

MGI phenotypes (gene matching)

abnormal corpus callosum morphology / MGI
absent corpus callosum / MGI
abnormal neuron morphology / MGI
abnormal axon guidance / MGI
no phenotypic analysis / MGI
digestive/alimentary phenotype / MGI
abnormal axon morphology / MGI
absent hippocampal commissure / MGI

Literature references

Functional analysis of candidate genes from genome-wide association studies of hearing.;Ingham Neil J, Rook Victoria, Di Domenico Francesca, James Elysia, Lewis Morag A, Girotto Giorgia, Buniello Annalisa, Steel Karen P, ;2020;Hearing research;387;107879; 31927188

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Dclk1tm1a(EUCOMM)Wtsi/WtsiOrl