C57BL/6NTac-Gmds^{tm1a(KOMP)Wtsi}/WtsiIeg

Status	Available to order
EMMA ID	EM:09706
International strain name	C57BL/6NTac-Gmds^{tm1a(KOMP)Wtsi}/WtsiIeg
Alternative name	EPD0034_5_H06
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Gmds^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Gmds
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0034_5_H06. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased hematocrit / IMPC
increased leukocyte cell number / IMPC
increased neutrophil cell number / IMPC
increased monocyte cell number / IMPC
increased granulocyte number / IMPC
abnormal cranium morphology / IMPC
abnormal snout morphology / IMPC
decreased body length / IMPC
decreased hemoglobin content / IMPC
decreased erythrocyte cell number / IMPC
abnormal bone structure / IMPC
decreased macrophage cell number / IMPC
decreased lean body mass / IMPC
decreased regulatory T cell number / IMPC
increased NK T cell number / IMPC
decreased NK cell number / IMPC
increased CD4-positive, alpha-beta T cell number / IMPC
decreased CD4-positive, alpha-beta T cell number / IMPC
increased CD8-positive, alpha-beta T cell number / IMPC
decreased CD8-positive, alpha-beta T cell number / IMPC
decreased plasmacytoid dendritic cell number / IMPC
decreased dendritic cell number / IMPC
increased gamma-delta T cell number / IMPC
increased circulating iron level / IMPC
decreased transitional stage T2 B cell number / IMPC
increased total body fat amount / IMPC
increased grip strength / IMPC
decreased circulating magnesium level / IMPC
decreased bone mineral content / IMPC
decreased CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
decreased effector memory CD8-positive, alpha-beta T cell number / IMPC
decreased alpha-beta T cell number / IMPC
decreased KLRG1-positive NK cell number / IMPC
decreased KLRG1+ CD8 alpha-beta T cell number / IMPC
increased memory-marker NK cell number / IMPC
decreased memory CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
increased memory CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
decreased CD8-positive, naive alpha-beta T cell number / IMPC
increased CD4-negative NK T cell number / IMPC
decreased memory-marker CD4-negative NK T cell number / IMPC
decreased CD4-positive NK T cell number / IMPC
decreased memory-marker CD4-positive NK T cell number / IMPC
increased immature NK cell number / IMPC
decreased mature NK cell number / IMPC
decreased Ly6C-positive mature NK cell number / IMPC
increased Ly6C-positive NK T cell number / IMPC
decreased T-helper cell number / IMPC

IMPC phenotypes (gene matching)

increased leukocyte cell number / IMPC
decreased circulating magnesium level / IMPC
decreased CD8-positive, naive alpha-beta T cell number / IMPC
decreased CD4-positive NK T cell number / IMPC
decreased T-helper cell number / IMPC
decreased plasmacytoid dendritic cell number / IMPC
decreased CD4-positive, alpha-beta T cell number / IMPC
decreased NK cell number / IMPC
decreased body length / IMPC
increased NK T cell number / IMPC
decreased KLRG1-positive NK cell number / IMPC
decreased dendritic cell number / IMPC
decreased bone mineral content / IMPC
increased CD4-positive, alpha-beta T cell number / IMPC
increased CD4-negative NK T cell number / IMPC
increased neutrophil cell number / IMPC
increased gamma-delta T cell number / IMPC
decreased memory CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
decreased regulatory T cell number / IMPC
decreased alpha-beta T cell number / IMPC
decreased mature NK cell number / IMPC
decreased CD8-positive, alpha-beta T cell number / IMPC
decreased KLRG1+ CD8 alpha-beta T cell number / IMPC
decreased transitional stage T2 B cell number / IMPC
abnormal bone structure / IMPC
decreased hemoglobin content / IMPC
decreased memory-marker CD4-negative NK T cell number / IMPC
decreased macrophage cell number / IMPC
increased memory-marker NK cell number / IMPC
decreased erythrocyte cell number / IMPC
decreased Ly6C-positive mature NK cell number / IMPC
increased total body fat amount / IMPC
increased memory CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
increased CD8-positive, alpha-beta T cell number / IMPC
decreased effector memory CD8-positive, alpha-beta T cell number / IMPC
decreased memory-marker CD4-positive NK T cell number / IMPC
increased Ly6C-positive NK T cell number / IMPC
decreased hematocrit / IMPC
abnormal snout morphology / IMPC
increased circulating iron level / IMPC
increased granulocyte number / IMPC
decreased CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
increased grip strength / IMPC
increased monocyte cell number / IMPC
increased immature NK cell number / IMPC
decreased lean body mass / IMPC
abnormal cranium morphology / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Gmdstm1a(KOMP)Wtsi/WtsiIeg