strain-details - INFRAFRONTIER

Information from provider

Status	Available to order
EMMA ID	EM:09704
International strain name	C57BL/6N-A^tm1Brd Chst11^{tm1a(KOMP)Wtsi}/WtsiFlmg
Alternative name	EPD0293_6_C11
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Chst11^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Chst11
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0293_6_C11. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Disease and phenotype information

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece

IMPC phenotypes (allele matching)

preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, complete penetrance / IMPC

MGI phenotypes (allele matching)

cleft palate / MGI
abnormal thymus morphology / MGI
abnormal Mullerian duct morphology / MGI
bicuspid aortic valve / MGI
cerebral arteriovenous malformation / MGI
double ureter / MGI
umbilical vein stenosis / MGI
ductus venosus stenosis / MGI
hepatic portal vein stenosis / MGI
abnormal perichondrial ossification / MGI
abnormal eye muscle topology / MGI
abnormal vitelline vein connection / MGI
blood in lymph vessels / MGI
abnormal vitelline vein topology / MGI
abnormal vertebral artery topology / MGI
additional anastomosis between intracranial vertebral arteries / MGI
embryo cyst / MGI

MGI phenotypes (gene matching)

short mandible / MGI
short maxilla / MGI
cleft palate / MGI
abnormal vertebrae morphology / MGI
abnormal cartilage morphology / MGI
abnormal long bone hypertrophic chondrocyte zone / MGI
domed cranium / MGI
short limbs / MGI
abnormal thymus morphology / MGI
respiratory distress / MGI
disproportionate dwarf / MGI
abnormal Mullerian duct morphology / MGI
small scapula / MGI
short nasal bone / MGI
abnormal vertebral arch morphology / MGI
decreased length of long bones / MGI
abnormal vertebral column morphology / MGI
abnormal talus morphology / MGI
abnormal phalanx morphology / MGI
abnormal epiphyseal plate morphology / MGI
increased diameter of long bones / MGI
small thoracic cage / MGI
bicuspid aortic valve / MGI
cerebral arteriovenous malformation / MGI
neonatal lethality, complete penetrance / MGI
double ureter / MGI
umbilical vein stenosis / MGI
ductus venosus stenosis / MGI
hepatic portal vein stenosis / MGI
abnormal perichondrial ossification / MGI
abnormal eye muscle topology / MGI
abnormal vitelline vein connection / MGI
blood in lymph vessels / MGI
abnormal vitelline vein topology / MGI
abnormal vertebral artery topology / MGI
additional anastomosis between intracranial vertebral arteries / MGI
embryo cyst / MGI
short facial bone / MGI
short ilium / MGI
wide ilium / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*

Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request

Conditional ready allele (tm1c allele), service fee and delivery time available upon request

Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

C57BL/6N-A^tm1Brd Chst11^{tm1a(KOMP)Wtsi}/WtsiFlmg

Information from provider

Information from EMMA

Disease and phenotype information

Information on how we integrate external resources can be found here

Availabilities

Practical information

Other EMMA strains

C57BL/6N-Atm1Brd Chst11tm1a(KOMP)Wtsi/WtsiFlmg

Information from provider

Information from EMMA

Disease and phenotype information

Information on how we integrate external resources can be found here

Availabilities

Practical information

Other EMMA strains

C57BL/6N-A^tm1Brd Chst11^{tm1a(KOMP)Wtsi}/WtsiFlmg