C57BL/6N-A^tm1Brd Clpp^{tm1a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:09701
International strain name	C57BL/6N-A^tm1Brd Clpp^{tm1a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0665_3_D05
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Clpp^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Clpp
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0665_3_D05. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Perrault syndrome / Orphanet_2855

IMPC phenotypes (allele matching)

decreased bone mineral density / IMPC
increased leukocyte cell number / IMPC
increased monocyte cell number / IMPC
tremors / IMPC
decreased body length / IMPC
male infertility / IMPC
female infertility / IMPC
increased hematocrit / IMPC
increased circulating serum albumin level / IMPC
increased circulating alkaline phosphatase level / IMPC
thrombocytopenia / IMPC
increased circulating glycerol level / IMPC
abnormal bone structure / IMPC
increased lean body mass / IMPC
decreased lean body mass / IMPC
abnormal auditory brainstem response / IMPC
increased B cell number / IMPC
increased circulating cholesterol level / IMPC
increased circulating aspartate transaminase level / IMPC
increased circulating bilirubin level / IMPC
decreased circulating glucose level / IMPC
increased mean corpuscular hemoglobin / IMPC
increased hemoglobin content / IMPC
decreased NK T cell number / IMPC
increased mature B cell number / IMPC
decreased gamma-delta T cell number / IMPC
increased circulating iron level / IMPC
increased total body fat amount / IMPC
decreased total body fat amount / IMPC
decreased red blood cell distribution width / IMPC
decreased circulating fructosamine level / IMPC
decreased bone mineral content / IMPC
decreased effector memory CD8-positive, alpha-beta T cell number / IMPC
increased KLRG1-positive NK cell number / IMPC
increased Ly6C high monocyte number / IMPC
increased Ly6C low monocyte number / IMPC
decreased memory-marker CD4-negative NK T cell number / IMPC
decreased CD4-positive NK T cell number / IMPC
decreased memory-marker CD4-positive NK T cell number / IMPC

IMPC phenotypes (gene matching)

thrombocytopenia / IMPC
increased circulating bilirubin level / IMPC
decreased lean body mass / IMPC
increased leukocyte cell number / IMPC
increased circulating cholesterol level / IMPC
increased mean corpuscular hemoglobin / IMPC
increased monocyte cell number / IMPC
increased Ly6C low monocyte number / IMPC
decreased bone mineral content / IMPC
decreased circulating fructosamine level / IMPC
increased lean body mass / IMPC
increased KLRG1-positive NK cell number / IMPC
increased circulating glycerol level / IMPC
decreased total body fat amount / IMPC
decreased NK T cell number / IMPC
decreased memory-marker CD4-positive NK T cell number / IMPC
decreased body length / IMPC
increased hemoglobin content / IMPC
increased total body fat amount / IMPC
increased mature B cell number / IMPC
decreased bone mineral density / IMPC
decreased circulating glucose level / IMPC
increased circulating serum albumin level / IMPC
increased B cell number / IMPC
abnormal bone structure / IMPC
tremors / IMPC
increased circulating iron level / IMPC
decreased effector memory CD8-positive, alpha-beta T cell number / IMPC
abnormal auditory brainstem response / IMPC
increased Ly6C high monocyte number / IMPC
female infertility / IMPC
decreased CD4-positive NK T cell number / IMPC
decreased red blood cell distribution width / IMPC
increased circulating aspartate transaminase level / IMPC
increased hematocrit / IMPC
decreased memory-marker CD4-negative NK T cell number / IMPC
increased circulating alkaline phosphatase level / IMPC
male infertility / IMPC
decreased gamma-delta T cell number / IMPC

MGI phenotypes (gene matching)

decreased cell proliferation / MGI
small ovary / MGI
small testis / MGI
arrest of spermatogenesis / MGI
dermatitis / MGI
decreased body length / MGI
hypoactivity / MGI
decreased startle reflex / MGI
abnormal T cell activation / MGI
male infertility / MGI
female infertility / MGI
abnormal oogenesis / MGI
premature death / MGI
decreased corpora lutea number / MGI
decreased vertical activity / MGI
azoospermia / MGI
early cellular replicative senescence / MGI
abnormal granulosa cell morphology / MGI
decreased susceptibility to weight gain / MGI
abnormal cellular respiration / MGI
preweaning lethality, incomplete penetrance / MGI
increased mitochondria size / MGI
disorganized mitochondrial cristae / MGI
abnormal mitochondrial chromosome morphology / MGI
abnormal seminiferous tubule epithelium morphology / MGI
increased embryonic tissue cell apoptosis / MGI
increased granulosa cell apoptosis / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Atm1Brd Clpptm1a(EUCOMM)Wtsi/WtsiH