C57BL/6N-A^tm1Brd Cxcr2^{tm1a(EUCOMM)Wtsi}/WtsiOulu

Status	Available to order
EMMA ID	EM:09700
International strain name	C57BL/6N-A^tm1Brd Cxcr2^{tm1a(EUCOMM)Wtsi}/WtsiOulu
Alternative name	EPD0664_3_D02
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cxcr2^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Cxcr2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0664_3_D02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Autosomal recessive severe congenital neutropenia due to CXCR2 deficiency / Orphanet_420699

IMPC phenotypes (allele matching)

increased circulating calcium level / IMPC
increased leukocyte cell number / IMPC
increased neutrophil cell number / IMPC
increased monocyte cell number / IMPC
increased granulocyte number / IMPC
increased anti-nuclear antigen antibody level / IMPC
increased eosinophil cell number / IMPC
decreased B cell number / IMPC
decreased T cell number / IMPC
improved glucose tolerance / IMPC
decreased circulating serum albumin level / IMPC
decreased circulating thyroxine level / IMPC
thrombocytosis / IMPC
increased circulating creatinine level / IMPC
decreased mean corpuscular hemoglobin / IMPC
increased circulating total protein level / IMPC
decreased CD8-positive, alpha-beta T cell number / IMPC
decreased pre-B cell number / IMPC
decreased mature B cell number / IMPC
decreased immature B cell number / IMPC
increased gamma-delta T cell number / IMPC
increased red blood cell distribution width / IMPC
decreased circulating fructosamine level / IMPC
increased circulating magnesium level / IMPC
increased CD4-positive, alpha-beta memory T cell number / IMPC
increased CD8-positive, alpha-beta memory T cell number / IMPC
increased effector memory CD8-positive, alpha-beta T cell number / IMPC
decreased pre-pro B cell number / IMPC
increased Ly6C high monocyte number / IMPC
increased Ly6C low monocyte number / IMPC
increased KLRG1+ CD8 alpha-beta T cell number / IMPC
increased KLRG1+ CD4 alpha-beta T cell number / IMPC
increased memory-marker gamma-delta T cell number / IMPC
increased memory-marker NK cell number / IMPC
increased memory-marker CD4-positive NK T cell number / IMPC
increased CD103-positive CD11b-low dendritic cell number / IMPC
increased effector memory T-helper cell number / IMPC

IMPC phenotypes (gene matching)

increased CD8-positive, alpha-beta memory T cell number / IMPC
increased monocyte cell number / IMPC
increased memory-marker gamma-delta T cell number / IMPC
increased eosinophil cell number / IMPC
increased neutrophil cell number / IMPC
decreased pre-pro B cell number / IMPC
increased circulating total protein level / IMPC
increased gamma-delta T cell number / IMPC
increased leukocyte cell number / IMPC
increased KLRG1+ CD4 alpha-beta T cell number / IMPC
increased circulating creatinine level / IMPC
increased circulating magnesium level / IMPC
improved glucose tolerance / IMPC
increased granulocyte number / IMPC
increased CD4-positive, alpha-beta memory T cell number / IMPC
increased CD103-positive CD11b-low dendritic cell number / IMPC
increased Ly6C high monocyte number / IMPC
decreased CD8-positive, alpha-beta T cell number / IMPC
increased effector memory T-helper cell number / IMPC
decreased mature B cell number / IMPC
increased circulating calcium level / IMPC
decreased T cell number / IMPC
decreased circulating thyroxine level / IMPC
increased effector memory CD8-positive, alpha-beta T cell number / IMPC
thrombocytosis / IMPC
decreased B cell number / IMPC
increased memory-marker NK cell number / IMPC
increased anti-nuclear antigen antibody level / IMPC
decreased mean corpuscular hemoglobin / IMPC
decreased pre-B cell number / IMPC
increased red blood cell distribution width / IMPC
increased Ly6C low monocyte number / IMPC
increased KLRG1+ CD8 alpha-beta T cell number / IMPC
increased memory-marker CD4-positive NK T cell number / IMPC
decreased circulating fructosamine level / IMPC
decreased circulating serum albumin level / IMPC
decreased immature B cell number / IMPC

MGI phenotypes (gene matching)

increased leukocyte cell number / MGI
increased neutrophil cell number / MGI
extramedullary hematopoiesis / MGI
increased granulocyte number / MGI
small liver / MGI
enlarged spleen / MGI
enlarged lymph nodes / MGI
abnormal myelination / MGI
decreased oligodendrocyte progenitor number / MGI
abnormal spinal cord morphology / MGI
decreased body size / MGI
decreased metastatic potential / MGI
abnormal myelopoiesis / MGI
abnormal respiratory mucosa goblet cell morphology / MGI
abnormal airway responsiveness / MGI
decreased airway responsiveness / MGI
abnormal spleen white pulp morphology / MGI
abnormal bone marrow morphology / MGI
increased susceptibility to bacterial infection / MGI
increased susceptibility to viral infection / MGI
abnormal leukocyte physiology / MGI
abnormal T cell physiology / MGI
abnormal neutrophil physiology / MGI
abnormal eosinophil physiology / MGI
increased IgE level / MGI
small adrenal glands / MGI
delayed wound healing / MGI
abnormal cytokine secretion / MGI
decreased susceptibility to induced arthritis / MGI
decreased tumor growth/size / MGI
abnormal tumor morphology / MGI
increased kidney weight / MGI
decreased kidney weight / MGI
increased seminal vesicle weight / MGI
increased epididymis weight / MGI
increased spleen weight / MGI
decreased spleen weight / MGI
decreased thymus weight / MGI
increased B cell number / MGI
increased susceptibility to parasitic infection / MGI
immune system phenotype / MGI
hematopoietic system phenotype / MGI
decreased susceptibility to fungal infection / MGI
abnormal T-helper 1 physiology / MGI
abnormal T-helper 2 physiology / MGI
corneal vascularization / MGI
abnormal NK T cell physiology / MGI
abnormal granulocyte differentiation / MGI
enlarged cervical lymph nodes / MGI
increased circulating interleukin-6 level / MGI
impaired neutrophil recruitment / MGI
impaired neutrophil chemotaxis / MGI
abnormal chemokine level / MGI
abnormal interleukin level / MGI
decreased sensitivity to induced morbidity/mortality / MGI
decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
myeloid hyperplasia / MGI
lymph node medullary cord hyperplasia / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Atm1Brd Cxcr2tm1a(EUCOMM)Wtsi/WtsiOulu