C57BL/6N-Dbn1^{tm1b(KOMP)Wtsi}/WtsiOulu

Status	Available to order
EMMA ID	EM:09623
International strain name	C57BL/6N-Dbn1^{tm1b(KOMP)Wtsi}/WtsiOulu
Alternative name	EPD0211_3_A05
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Dbn1^{tm1b(KOMP)Wtsi}
Gene/Transgene symbol	Dbn1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0211_3_A05. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele using a cell permeable HTN-Cre as described in doi:10.1007/s11248-013-9764-x. Click here for more information on KOMP final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland

Disease and phenotype information

IMPC phenotypes (allele matching)

abnormal vertebrae morphology / IMPC
abnormal heart morphology / IMPC
enlarged heart / IMPC
microcephaly / IMPC
syndactyly / IMPC
abnormal liver morphology / IMPC
no spontaneous movement / IMPC
increased exploration in new environment / IMPC
unresponsive to tactile stimuli / IMPC
increased circulating phosphate level / IMPC
abnormal embryo size / IMPC
decreased circulating free fatty acids level / IMPC
decreased vertical activity / IMPC
increased circulating alkaline phosphatase level / IMPC
increased circulating chloride level / IMPC
increased lean body mass / IMPC
decreased circulating cholesterol level / IMPC
decreased circulating serum albumin level / IMPC
increased circulating creatinine level / IMPC
increased CD8-positive, alpha-beta T cell number / IMPC
decreased mean platelet volume / IMPC
increased prepulse inhibition / IMPC
decreased total body fat amount / IMPC
decreased grip strength / IMPC
increased red blood cell distribution width / IMPC
increased circulating magnesium level / IMPC
increased bone mineral content / IMPC
preweaning lethality, complete penetrance / IMPC
preweaning lethality, incomplete penetrance / IMPC
decreased total retina thickness / IMPC
increased fasting circulating glucose level / IMPC

IMPC phenotypes (gene matching)

increased red blood cell distribution width / IMPC
microcephaly / IMPC
decreased body weight / IMPC
increased lean body mass / IMPC
preweaning lethality, complete penetrance / IMPC
enlarged heart / IMPC
increased bone mineral content / IMPC
decreased total body fat amount / IMPC
hyperactivity / IMPC
syndactyly / IMPC
decreased total retina thickness / IMPC
abnormal allantois morphology / IMPC
decreased mean platelet volume / IMPC
decreased circulating serum albumin level / IMPC
preweaning lethality, incomplete penetrance / IMPC
unresponsive to tactile stimuli / IMPC
abnormal heart morphology / IMPC
increased CD8-positive, alpha-beta T cell number / IMPC
decreased circulating fructosamine level / IMPC
increased leukocyte cell number / IMPC
decreased grip strength / IMPC
increased prepulse inhibition / IMPC
abnormal coat appearance / IMPC
abnormal embryo turning / IMPC
abnormal chorioallantoic fusion / IMPC
increased fasting circulating glucose level / IMPC
abnormal vertebrae morphology / IMPC
abnormal embryo size / IMPC
no spontaneous movement / IMPC
increased exploration in new environment / IMPC
increased circulating chloride level / IMPC
decreased circulating cholesterol level / IMPC
decreased circulating free fatty acids level / IMPC
increased circulating phosphate level / IMPC
increased circulating alkaline phosphatase level / IMPC
decreased circulating HDL cholesterol level / IMPC
decreased vertical activity / IMPC
increased circulating magnesium level / IMPC
abnormal liver morphology / IMPC
increased circulating creatinine level / IMPC

MGI phenotypes (allele matching)

enlarged liver sinusoidal spaces / MGI
abnormal forebrain morphology / MGI
abnormal telencephalon morphology / MGI
abnormal brain white matter morphology / MGI
abnormal brain internal capsule morphology / MGI
abnormal cervical rib / MGI
abnormal hypoglossal canal morphology / MGI
absent hypoglossal canal / MGI
thin hypoglossal nerve / MGI
absent hypoglossal nerve / MGI
abnormal brachial plexus formation / MGI
abnormal Wolffian duct connection / MGI

MGI phenotypes (gene matching)

decreased circulating LDL cholesterol level / MGI
decreased circulating HDL cholesterol level / MGI
decreased circulating calcium level / MGI
enlarged liver sinusoidal spaces / MGI
abnormal forebrain morphology / MGI
abnormal telencephalon morphology / MGI
abnormal hippocampus morphology / MGI
decreased body weight / MGI
impaired synaptic plasticity / MGI
decreased mean corpuscular volume / MGI
decreased circulating alanine transaminase level / MGI
no phenotypic analysis / MGI
increased circulating chloride level / MGI
decreased circulating cholesterol level / MGI
decreased circulating serum albumin level / MGI
decreased circulating total protein level / MGI
decreased circulating aspartate transaminase level / MGI
abnormal brain white matter morphology / MGI
abnormal brain internal capsule morphology / MGI
abnormal cervical rib / MGI
impaired cued conditioning behavior / MGI
abnormal dendritic spine morphology / MGI
decreased total body fat amount / MGI
decreased circulating fructosamine level / MGI
abnormal hypoglossal canal morphology / MGI
absent hypoglossal canal / MGI
thin hypoglossal nerve / MGI
absent hypoglossal nerve / MGI
abnormal brachial plexus formation / MGI
abnormal Wolffian duct connection / MGI

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Dbn1tm1b(KOMP)Wtsi/WtsiOulu