B6.129-Slc17a6^tm1Kldr/Kctt

Status	Available to order
EMMA ID	EM:09588
International strain name	B6.129-Slc17a6^tm1Kldr/Kctt
Alternative name	VGLUT2-Lox-C57Bl/6-Backcross
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Slc17a6^tm1Kldr,
Gene/Transgene symbol	Slc17a6

Information from provider

Provider	Åsa Mackenzie
Provider affiliation	Inst. för Neurovetenskap
Genetic information	The Slc17a6 (Vglut2) gene is flanked by loxP sites.
Phenotypic information	Homozygous: The mice have no known phenotype. Heterozygous: The mice have no known phenotype
Breeding history	The Vglut2-lox mouse has been generated on a mixed background, C57BL/6 and 129/Sv, and then been bred to a pure C57BL/6 background by performing 10 backcrosses to C57BL/6. The C57BL/6-backcrossed line was archived in EMMA. Published data refers to the mixed genetic background.
References	Vesicular glutamate transporter 2 is required for central respiratory rhythm generation but not for locomotor central pattern generation.;Wallén-Mackenzie Asa, Gezelius Henrik, Thoby-Brisson Muriel, Nygård Anna, Enjin Anders, Fujiyama Fumino, Fortin Gilles, Kullander Klas, ;2006;The Journal of neuroscience : the official journal of the Society for Neuroscience;26;12294-307; 17122055 Restricted cortical and amygdaloid removal of vesicular glutamate transporter 2 in preadolescent mice impacts dopaminergic activity and neuronal circuitry of higher brain function.;Wallén-Mackenzie Asa, Nordenankar Karin, Fejgin Kim, Lagerström Malin C, Emilsson Lina, Fredriksson Robert, Wass Caroline, Andersson Daniel, Egecioglu Emil, Andersson My, Strandberg Joakim, Lindhe Orjan, Schiöth Helgi B, Chergui Karima, Hanse Eric, Långström Bengt, Fredriksson Anders, Svensson Lennart, Roman Erika, Kullander Klas, ;2009;The Journal of neuroscience : the official journal of the Society for Neuroscience;29;2238-51; 19228977 VGLUT2 in dopamine neurons is required for psychostimulant-induced behavioral activation.;Birgner Carolina, Nordenankar Karin, Lundblad Martin, Mendez José Alfredo, Smith Casey, le Grevès Madeleine, Galter Dagmar, Olson Lars, Fredriksson Anders, Trudeau Louis-Eric, Kullander Klas, Wallén-Mackenzie Asa, ;2010;Proceedings of the National Academy of Sciences of the United States of America;107;389-94; 20018672 A sensory subpopulation depends on vesicular glutamate transporter 2 for mechanical pain, and together with substance P, inflammatory pain.;Lagerström Malin C, Rogoz Katarzyna, Abrahamsen Bjarke, Lind Anne-Li, Olund Caroline, Smith Casey, Mendez José Alfredo, Wallén-Mackenzie Åsa, Wood John N, Kullander Klas, ;2011;Proceedings of the National Academy of Sciences of the United States of America;108;5789-94; 21415372 VGLUT2-dependent sensory neurons in the TRPV1 population regulate pain and itch.;Lagerström Malin C, Rogoz Katarzyna, Abrahamsen Bjarke, Persson Emma, Reinius Björn, Nordenankar Karin, Olund Caroline, Smith Casey, Mendez José Alfredo, Chen Zhou-Feng, Wood John N, Wallén-Mackenzie Asa, Kullander Klas, ;2010;Neuron;68;529-42; 21040852 Enhanced sucrose and cocaine self-administration and cue-induced drug seeking after loss of VGLUT2 in midbrain dopamine neurons in mice.;Alsiö Johan, Nordenankar Karin, Arvidsson Emma, Birgner Carolina, Mahmoudi Souha, Halbout Briac, Smith Casey, Fortin Guillaume M, Olson Lars, Descarries Laurent, Trudeau Louis-Éric, Kullander Klas, Lévesque Daniel, Wallén-Mackenzie Asa, ;2011;The Journal of neuroscience : the official journal of the Society for Neuroscience;31;12593-603; 21880920 Limiting glutamate transmission in a Vglut2-expressing subpopulation of the subthalamic nucleus is sufficient to cause hyperlocomotion.;Schweizer Nadine, Pupe Stéfano, Arvidsson Emma, Nordenankar Karin, Smith-Anttila Casey J A, Mahmoudi Souha, Andrén Anna, Dumas Sylvie, Rajagopalan Aparna, Lévesque Daniel, Leão Richardson N, Wallén-Mackenzie Åsa, ;2014;Proceedings of the National Academy of Sciences of the United States of America;111;7837-42; 24821804 Increased hippocampal excitability and impaired spatial memory function in mice lacking VGLUT2 selectively in neurons defined by tyrosine hydroxylase promoter activity.;Nordenankar Karin, Smith-Anttila Casey J A, Schweizer Nadine, Viereckel Thomas, Birgner Carolina, Mejia-Toiber Jana, Morales Marisela, Leao Richardson N, Wallén-Mackenzie Åsa, ;2015;Brain structure & function;220;2171-90; 24802380
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Karolinska Institutet, Stockholm, Sweden
Animals used for archiving	heterozygous C57BL/6J

Literature references

Vesicular glutamate transporter 2 is required for central respiratory rhythm generation but not for locomotor central pattern generation.;Wallén-Mackenzie Asa, Gezelius Henrik, Thoby-Brisson Muriel, Nygård Anna, Enjin Anders, Fujiyama Fumino, Fortin Gilles, Kullander Klas, ;2006;The Journal of neuroscience : the official journal of the Society for Neuroscience;26;12294-307; 17122055
Restricted cortical and amygdaloid removal of vesicular glutamate transporter 2 in preadolescent mice impacts dopaminergic activity and neuronal circuitry of higher brain function.;Wallén-Mackenzie Asa, Nordenankar Karin, Fejgin Kim, Lagerström Malin C, Emilsson Lina, Fredriksson Robert, Wass Caroline, Andersson Daniel, Egecioglu Emil, Andersson My, Strandberg Joakim, Lindhe Orjan, Schiöth Helgi B, Chergui Karima, Hanse Eric, Långström Bengt, Fredriksson Anders, Svensson Lennart, Roman Erika, Kullander Klas, ;2009;The Journal of neuroscience : the official journal of the Society for Neuroscience;29;2238-51; 19228977
VGLUT2 in dopamine neurons is required for psychostimulant-induced behavioral activation.;Birgner Carolina, Nordenankar Karin, Lundblad Martin, Mendez José Alfredo, Smith Casey, le Grevès Madeleine, Galter Dagmar, Olson Lars, Fredriksson Anders, Trudeau Louis-Eric, Kullander Klas, Wallén-Mackenzie Asa, ;2010;Proceedings of the National Academy of Sciences of the United States of America;107;389-94; 20018672
A sensory subpopulation depends on vesicular glutamate transporter 2 for mechanical pain, and together with substance P, inflammatory pain.;Lagerström Malin C, Rogoz Katarzyna, Abrahamsen Bjarke, Lind Anne-Li, Olund Caroline, Smith Casey, Mendez José Alfredo, Wallén-Mackenzie Åsa, Wood John N, Kullander Klas, ;2011;Proceedings of the National Academy of Sciences of the United States of America;108;5789-94; 21415372
VGLUT2-dependent sensory neurons in the TRPV1 population regulate pain and itch.;Lagerström Malin C, Rogoz Katarzyna, Abrahamsen Bjarke, Persson Emma, Reinius Björn, Nordenankar Karin, Olund Caroline, Smith Casey, Mendez José Alfredo, Chen Zhou-Feng, Wood John N, Wallén-Mackenzie Asa, Kullander Klas, ;2010;Neuron;68;529-42; 21040852
Enhanced sucrose and cocaine self-administration and cue-induced drug seeking after loss of VGLUT2 in midbrain dopamine neurons in mice.;Alsiö Johan, Nordenankar Karin, Arvidsson Emma, Birgner Carolina, Mahmoudi Souha, Halbout Briac, Smith Casey, Fortin Guillaume M, Olson Lars, Descarries Laurent, Trudeau Louis-Éric, Kullander Klas, Lévesque Daniel, Wallén-Mackenzie Asa, ;2011;The Journal of neuroscience : the official journal of the Society for Neuroscience;31;12593-603; 21880920
Limiting glutamate transmission in a Vglut2-expressing subpopulation of the subthalamic nucleus is sufficient to cause hyperlocomotion.;Schweizer Nadine, Pupe Stéfano, Arvidsson Emma, Nordenankar Karin, Smith-Anttila Casey J A, Mahmoudi Souha, Andrén Anna, Dumas Sylvie, Rajagopalan Aparna, Lévesque Daniel, Leão Richardson N, Wallén-Mackenzie Åsa, ;2014;Proceedings of the National Academy of Sciences of the United States of America;111;7837-42; 24821804
Increased hippocampal excitability and impaired spatial memory function in mice lacking VGLUT2 selectively in neurons defined by tyrosine hydroxylase promoter activity.;Nordenankar Karin, Smith-Anttila Casey J A, Schweizer Nadine, Viereckel Thomas, Birgner Carolina, Mejia-Toiber Jana, Morales Marisela, Leao Richardson N, Wallén-Mackenzie Åsa, ;2015;Brain structure & function;220;2171-90; 24802380

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

B6.129-Slc17a6tm1Kldr/Kctt