C57BL/6N-A^tm1Brd Dph6^{tm1a(KOMP)Wtsi}/WtsiFlmg

Status	Available to order
EMMA ID	EM:09484
Citation information	RRID:IMSR_EM:09484 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-A^tm1Brd Dph6^{tm1a(KOMP)Wtsi}/WtsiFlmg
Alternative name	EPD0702_3_E03
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Dph6^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Dph6
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0702_3_E03. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased hematocrit / IMPC
increased monocyte cell number / IMPC
increased granulocyte number / IMPC
decreased body length / IMPC
abnormal coat/hair pigmentation / IMPC
decreased hemoglobin content / IMPC
decreased erythrocyte cell number / IMPC
vertebral transformation / IMPC
increased circulating creatinine level / IMPC
increased blood urea nitrogen level / IMPC
decreased NK T cell number / IMPC
increased CD4-positive, alpha-beta T cell number / IMPC
decreased CD8-positive, alpha-beta T cell number / IMPC
increased plasma cell number / IMPC
increased dendritic cell number / IMPC
increased marginal zone B cell number / IMPC
decreased mature B cell number / IMPC
increased gamma-delta T cell number / IMPC
increased plasmacytoid dendritic cell number / IMPC
increased red blood cell distribution width / IMPC
increased circulating magnesium level / IMPC
increased CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
increased CD4-positive, alpha-beta memory T cell number / IMPC
decreased effector memory CD8-positive, alpha-beta T cell number / IMPC
increased memory-marker gamma-delta T cell number / IMPC
increased CD5-positive gamma-delta T cell number / IMPC
increased memory CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
increased KLRG1-positive CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
decreased CD8-positive, naive alpha-beta T cell number / IMPC
increased memory-marker CD4-negative NK T cell number / IMPC
increased CD11b-low dendritic cell number / IMPC
decreased Ly6C-positive mature NK cell number / IMPC
decreased T-helper cell number / IMPC
increased effector memory T-helper cell number / IMPC
increased KLRG1-positive T-helper cell number / IMPC

IMPC phenotypes (gene matching)

increased CD4-positive, alpha-beta memory T cell number / IMPC
decreased CD8-positive, naive alpha-beta T cell number / IMPC
increased KLRG1-positive CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
increased plasma cell number / IMPC
abnormal coat/hair pigmentation / IMPC
increased marginal zone B cell number / IMPC
increased circulating creatinine level / IMPC
increased CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
decreased hematocrit / IMPC
decreased body length / IMPC
increased gamma-delta T cell number / IMPC
decreased CD8-positive, alpha-beta T cell number / IMPC
increased CD11b-low dendritic cell number / IMPC
increased CD4-positive, alpha-beta T cell number / IMPC
increased plasmacytoid dendritic cell number / IMPC
increased memory-marker gamma-delta T cell number / IMPC
increased granulocyte number / IMPC
increased memory CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
increased monocyte cell number / IMPC
increased KLRG1-positive T-helper cell number / IMPC
increased dendritic cell number / IMPC
increased effector memory T-helper cell number / IMPC
decreased mature B cell number / IMPC
increased circulating magnesium level / IMPC
vertebral transformation / IMPC
decreased erythrocyte cell number / IMPC
increased CD5-positive gamma-delta T cell number / IMPC
increased red blood cell distribution width / IMPC
decreased T-helper cell number / IMPC
increased memory-marker CD4-negative NK T cell number / IMPC
decreased effector memory CD8-positive, alpha-beta T cell number / IMPC
decreased NK T cell number / IMPC
increased blood urea nitrogen level / IMPC
decreased Ly6C-positive mature NK cell number / IMPC
decreased hemoglobin content / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

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C57BL/6N-Atm1Brd Dph6tm1a(KOMP)Wtsi/WtsiFlmg