C57BL/6N-A^tm1Brd Sgms1^{tm1a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:09471
Citation information	RRID:IMSR_EM:09471 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-A^tm1Brd Sgms1^{tm1a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0725_2_G05
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Sgms1^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Sgms1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0725_2_G05. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	A new mutation of Sgms1 causes gradual hearing loss associated with a reduced endocochlear potential.;Chen Jing, Lewis Morag A, Wai Alisa, Yin Lucia, Dawson Sally J, Ingham Neil J, Steel Karen P, ;2024;Hearing research;451;109091; 39067415

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)

tremors / IMPC
male infertility / IMPC
increased mean platelet volume / IMPC
increased circulating alkaline phosphatase level / IMPC
thrombocytopenia / IMPC
increased lean body mass / IMPC
increased circulating creatinine level / IMPC
decreased mean corpuscular hemoglobin / IMPC
decreased total body fat amount / IMPC
increased grip strength / IMPC
increased circulating magnesium level / IMPC

IMPC phenotypes (gene matching)

decreased mean corpuscular hemoglobin / IMPC
increased grip strength / IMPC
increased circulating alkaline phosphatase level / IMPC
increased mean platelet volume / IMPC
male infertility / IMPC
increased lean body mass / IMPC
decreased total body fat amount / IMPC
thrombocytopenia / IMPC
tremors / IMPC
increased circulating creatinine level / IMPC
increased circulating magnesium level / IMPC

MGI phenotypes (gene matching)

decreased body weight / MGI
hyperglycemia / MGI
decreased white adipose tissue amount / MGI
premature death / MGI
decreased circulating insulin level / MGI
increased insulin sensitivity / MGI
abnormal cytokine secretion / MGI
decreased insulin secretion / MGI
oxidative stress / MGI
abnormal pancreatic islet morphology / MGI
impaired glucose tolerance / MGI
homeostasis/metabolism phenotype / MGI
decreased circulating tumor necrosis factor level / MGI
decreased tumor necrosis factor secretion / MGI
decreased circulating interleukin-6 level / MGI
decreased interleukin-6 secretion / MGI
abnormal cytokine level / MGI
abnormal sphingomyelin level / MGI
abnormal ceramide level / MGI

Literature references

A new mutation of Sgms1 causes gradual hearing loss associated with a reduced endocochlear potential.;Chen Jing, Lewis Morag A, Wai Alisa, Yin Lucia, Dawson Sally J, Ingham Neil J, Steel Karen P, ;2024;Hearing research;451;109091; 39067415

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Atm1Brd Sgms1tm1a(EUCOMM)Wtsi/WtsiH