STOCK Flt4^tm2.1Ali/Oulu

Status	Available to order
EMMA ID	EM:09463
International strain name	STOCK Flt4^tm2.1Ali/Oulu
Alternative name	Vegfr3flox/flox
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Flt4^tm2.1Ali
Gene/Transgene symbol	Flt4

Information from provider

Provider	Kari Alitalo
Provider affiliation	Translational Cancer Biology Program, Wihuri Research Institute, University of Helsinki
Genetic information	Vegfr3 conditional mouse
Phenotypic information	Homozygous: Homozygous mice for this allele are viable and fertile, and do not display any pathological phenotype. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have the first exon and part of the first intron of the Vegfr3 mouse gene deleted in the cre-expressing tissues. Heterozygous: Heterozygous mice for this allele are viable and fertile, and do not display any pathological phenotype.
References	Deletion of vascular endothelial growth factor C (VEGF-C) and VEGF-D is not equivalent to VEGF receptor 3 deletion in mouse embryos.;Haiko Paula, Makinen Taija, Keskitalo Salla, Taipale Jussi, Karkkainen Marika J, Baldwin Megan E, Stacker Steven A, Achen Marc G, Alitalo Kari, ;2008;Molecular and cellular biology;28;4843-50; 18519586 VEGFR-3 controls tip to stalk conversion at vessel fusion sites by reinforcing Notch signalling.;Tammela Tuomas, Zarkada Georgia, Nurmi Harri, Jakobsson Lars, Heinolainen Krista, Tvorogov Denis, Zheng Wei, Franco Claudio A, Murtomäki Aino, Aranda Evelyn, Miura Naoyuki, Ylä-Herttuala Seppo, Fruttiger Marcus, Mäkinen Taija, Eichmann Anne, Pollard Jeffrey W, Gerhardt Holger, Alitalo Kari, ;2011;Nature cell biology;13;1202-13; 21909098 VEGFR3 does not sustain retinal angiogenesis without VEGFR2.;Zarkada Georgia, Heinolainen Krista, Makinen Taija, Kubota Yoshiaki, Alitalo Kari, ;2015;Proceedings of the National Academy of Sciences of the United States of America;112;761-6; 25561555
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	University of Oulu, Oulu, Finland
Animals used for archiving	heterozygous C57BL/6, wild-type C57BL/6NCrl
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Tetralogy of Fallot / Orphanet_3303
Milroy disease / Orphanet_79452

MGI phenotypes (gene matching)

abnormal adipose tissue distribution / MGI
abnormal vascular development / MGI
abnormal intestine morphology / MGI
abnormal liver morphology / MGI
thick dermal layer / MGI
pale yolk sac / MGI
edema / MGI
pericardial edema / MGI
abnormal lymphatic vessel morphology / MGI
postnatal lethality / MGI
no abnormal phenotype detected / MGI
hydrops fetalis / MGI
no phenotypic analysis / MGI
extremity edema / MGI
lymphedema / MGI
abnormal lymph circulation / MGI
embryonic growth retardation / MGI
abnormal vitelline vascular remodeling / MGI
abnormal anterior cardinal vein morphology / MGI
abnormal dorsal aorta morphology / MGI
hemopericardium / MGI
cardiovascular system phenotype / MGI
reproductive system phenotype / MGI
abnormal lymphangiogenesis / MGI
absent lymphatic vessels / MGI
neonatal lethality, complete penetrance / MGI
prenatal lethality, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
chylous ascites / MGI
abnormal perineural vascular plexus morphology / MGI

Literature references

Deletion of vascular endothelial growth factor C (VEGF-C) and VEGF-D is not equivalent to VEGF receptor 3 deletion in mouse embryos.;Haiko Paula, Makinen Taija, Keskitalo Salla, Taipale Jussi, Karkkainen Marika J, Baldwin Megan E, Stacker Steven A, Achen Marc G, Alitalo Kari, ;2008;Molecular and cellular biology;28;4843-50; 18519586
VEGFR-3 controls tip to stalk conversion at vessel fusion sites by reinforcing Notch signalling.;Tammela Tuomas, Zarkada Georgia, Nurmi Harri, Jakobsson Lars, Heinolainen Krista, Tvorogov Denis, Zheng Wei, Franco Claudio A, Murtomäki Aino, Aranda Evelyn, Miura Naoyuki, Ylä-Herttuala Seppo, Fruttiger Marcus, Mäkinen Taija, Eichmann Anne, Pollard Jeffrey W, Gerhardt Holger, Alitalo Kari, ;2011;Nature cell biology;13;1202-13; 21909098
VEGFR3 does not sustain retinal angiogenesis without VEGFR2.;Zarkada Georgia, Heinolainen Krista, Makinen Taija, Kubota Yoshiaki, Alitalo Kari, ;2015;Proceedings of the National Academy of Sciences of the United States of America;112;761-6; 25561555
VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.;Lin Qiu-Yue, Zhang Yun-Long, Bai Jie, Liu Jin-Qiu, Li Hui-Hua, ;2021;Clinical and translational medicine;11;e374; 33783987
Angiotensin II Induces Cardiac Edema and Hypertrophic Remodeling through Lymphatic-Dependent Mechanisms.;Bai Jie, Yin Liangqingqing, Yu Wei-Jia, Zhang Yun-Long, Lin Qiu-Yue, Li Hui-Hua, ;2022;Oxidative medicine and cellular longevity;2022;5044046; 35222798

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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STOCK Flt4tm2.1Ali/Oulu