C57BL/6N-A^tm1Brd Polb^{tm1a(KOMP)Wtsi}/WtsiOrl

Status	Available to order
EMMA ID	EM:09391
International strain name	C57BL/6N-A^tm1Brd Polb^{tm1a(KOMP)Wtsi}/WtsiOrl
Alternative name	EPD0663_1_B01
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Polb^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Polb
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0663_1_B01. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6N

Disease and phenotype information

IMPC phenotypes (allele matching)

increased mean platelet volume / IMPC
increased lean body mass / IMPC
decreased total body fat amount / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, complete penetrance / IMPC
increased mean platelet volume / IMPC
increased lean body mass / IMPC
decreased total body fat amount / IMPC

MGI phenotypes (allele matching)

abnormal interatrial septum morphology / MGI
adrenal gland hypoplasia / MGI
abnormal forebrain morphology / MGI
abnormal cerebral cortex morphology / MGI
abnormal hindbrain morphology / MGI
small superior cervical ganglion / MGI
abnormal vagus ganglion morphology / MGI
small superior vagus ganglion / MGI
dermal cysts / MGI
small thyroid gland / MGI
abnormal cervical vertebrae morphology / MGI
abnormal eye muscle morphology / MGI
abnormal direction of heart looping / MGI
abnormal optic cup morphology / MGI
abnormal cervical atlas morphology / MGI
fusion of vertebral arches / MGI
absent stapedial artery / MGI
eye hemorrhage / MGI
dorsal root ganglion hypoplasia / MGI
abnormal brain internal capsule morphology / MGI
dorsal root ganglion hyperplasia / MGI
abnormal cervical rib / MGI
thin oculomotor nerve / MGI
thin facial nerve / MGI
thin splanchnic nerve / MGI
thin hypoglossal nerve / MGI
abnormal hypoglossal nerve topology / MGI
abnormal lymphatic vessel topology / MGI
subcutaneous edema / MGI
abnormal Wolffian duct topology / MGI
abnormal vitelline vein connection / MGI
trigeminal neuroma / MGI
thin motoric part of trigeminal nerve / MGI
retro-esophageal left subclavian artery / MGI
reduced sympathetic cervical ganglion size / MGI
inverse situs of great intrathoracic arteries / MGI
abnormal vertebral artery topology / MGI
absent extracranial vertebral artery segment / MGI
abnormal Wolffian duct connection / MGI

MGI phenotypes (gene matching)

abnormal interatrial septum morphology / MGI
decreased cell proliferation / MGI
adrenal gland hypoplasia / MGI
abnormal forebrain morphology / MGI
abnormal cerebral cortex morphology / MGI
abnormal hindbrain morphology / MGI
small superior cervical ganglion / MGI
abnormal vagus ganglion morphology / MGI
small superior vagus ganglion / MGI
atelectasis / MGI
dermatitis / MGI
dermal cysts / MGI
decreased body size / MGI
cyanosis / MGI
salivary gland inflammation / MGI
respiratory failure / MGI
abnormal reflex / MGI
abnormal pulmonary alveolus morphology / MGI
abnormal spleen germinal center morphology / MGI
increased IgG level / MGI
glomerulonephritis / MGI
small thyroid gland / MGI
abnormal cervical vertebrae morphology / MGI
abnormal cell cycle / MGI
increased neuron apoptosis / MGI
abnormal eye muscle morphology / MGI
pallor / MGI
lymph node inflammation / MGI
increased hepatocyte apoptosis / MGI
embryonic growth retardation / MGI
abnormal direction of heart looping / MGI
abnormal optic cup morphology / MGI
abnormal cervical atlas morphology / MGI
fusion of vertebral arches / MGI
absent stapedial artery / MGI
increased anti-nuclear antigen antibody level / MGI
increased susceptibility to systemic lupus erythematosus / MGI
abnormal somatic hypermutation frequency / MGI
eye hemorrhage / MGI
thin cerebral cortex / MGI
dorsal root ganglion hypoplasia / MGI
increased T-helper 2 cell number / MGI
abnormal brain internal capsule morphology / MGI
increased germinal center B cell number / MGI
increased spleen germinal center number / MGI
dorsal root ganglion hyperplasia / MGI
enlarged cervical lymph nodes / MGI
abnormal immunoglobulin heavy chain V(D)J recombination / MGI
chromosomal instability / MGI
abnormal cervical rib / MGI
increased sensitivity to induced cell death / MGI
increased splenocyte apoptosis / MGI
enlarged salivary gland / MGI
decreased birth weight / MGI
abnormal base-excision repair / MGI
abnormal neuron differentiation / MGI
neonatal lethality, complete penetrance / MGI
neonatal lethality, incomplete penetrance / MGI
perinatal lethality, complete penetrance / MGI
prenatal lethality, incomplete penetrance / MGI
increased kidney apoptosis / MGI
decreased fibroblast proliferation / MGI
increased embryonic tissue cell apoptosis / MGI
thin oculomotor nerve / MGI
thin facial nerve / MGI
thin splanchnic nerve / MGI
thin hypoglossal nerve / MGI
abnormal hypoglossal nerve topology / MGI
abnormal lymphatic vessel topology / MGI
subcutaneous edema / MGI
abnormal Wolffian duct topology / MGI
abnormal vitelline vein connection / MGI
trigeminal neuroma / MGI
thin motoric part of trigeminal nerve / MGI
retro-esophageal left subclavian artery / MGI
reduced sympathetic cervical ganglion size / MGI
inverse situs of great intrathoracic arteries / MGI
abnormal vertebral artery topology / MGI
absent extracranial vertebral artery segment / MGI
abnormal Wolffian duct connection / MGI
persistent trigeminal artery / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

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C57BL/6N-Atm1Brd Polbtm1a(KOMP)Wtsi/WtsiOrl