C57BL/6NTac-Rbl1^{tm1a(EUCOMM)Hmgu}/Ieg

Status	Available to order
EMMA ID	EM:09370
International strain name	C57BL/6NTac-Rbl1^{tm1a(EUCOMM)Hmgu}/Ieg
Alternative name	HEPD0864_4_C08
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Rbl1^{tm1a(EUCOMM)Hmgu}
Gene/Transgene symbol	Rbl1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0864_4_C08. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6NTacUSA

Disease and phenotype information

IMPC phenotypes (gene matching)

improved glucose tolerance / IMPC
abnormal kidney morphology / IMPC
shortened QRS complex duration / IMPC
preweaning lethality, incomplete penetrance / IMPC

MGI phenotypes (gene matching)

extramedullary hematopoiesis / MGI
increased bone marrow cell number / MGI
increased cell proliferation / MGI
spontaneous skin ulceration / MGI
decreased body weight / MGI
decreased body size / MGI
postnatal growth retardation / MGI
decreased white adipose tissue amount / MGI
abnormal inflammatory response / MGI
premature death / MGI
abnormal muscle physiology / MGI
abnormal definitive hematopoiesis / MGI
no abnormal phenotype detected / MGI
abnormal spleen red pulp morphology / MGI
hepatic steatosis / MGI
abnormal white adipose tissue morphology / MGI
no phenotypic analysis / MGI
abnormal postnatal subventricular zone morphology / MGI
abnormal neuronal precursor proliferation / MGI
increased neuronal precursor cell number / MGI
increased oxygen consumption / MGI
cellular phenotype / MGI
decreased percent body fat/body weight / MGI
abnormal common myeloid progenitor cell morphology / MGI
increased diameter of long bones / MGI
decreased total fat pad weight / MGI
decreased white fat cell size / MGI
decreased gonadal fat pad weight / MGI
decreased inguinal fat pad weight / MGI
decreased interscapular fat pad weight / MGI
integument phenotype / MGI
postnatal lethality, incomplete penetrance / MGI
increased fibroblast proliferation / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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