B6;D-Tg(tetO-GFP,-lacZ)G3Rsp/Kctt

Status	Available to order
EMMA ID	EM:09285
Citation information	RRID:IMSR_EM:09285 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;D-Tg(tetO-GFP,-lacZ)G3Rsp/Kctt
Alternative name	TgTetO/GFP-lacZ (lab name B6xG3
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(tetO-GFP,-lacZ)G3Rsp
Gene/Transgene symbol	Tg(tetO-GFP,-lacZ)G3Rsp

Information from provider

Provider	Rolf Sprengel
Provider affiliation	Molecular Neurobiology, Max Planck Institute for Medical Research
Genetic information	A transgene for enhanced GFP together with a lacZ reporter gene is under the control of the tTA dependent bidirectional synthetic promoter.
Phenotypic information	Homozygous: Not known Heterozygous: When the GFP and lacZ transgene is activated by tTA, the cells that express tTA can be visualised by GFP fluorescence or beta-galactosidase activity.
Breeding history	Embryos from mating hemizygous transgenic TgTetO/GFP-lacZ males and C57BL/6N females were harvested and 251 embryos were cryoconserved in January 2003.
References	A GFP-equipped bidirectional expression module well suited for monitoring tetracycline-regulated gene expression in mouse.;Krestel H E, Mayford M, Seeburg P H, Sprengel R, ;2001;Nucleic acids research;29;E39; 11266574 Neuronal co-expression of EGFP and beta-galactosidase in mice causes neuropathology and premature death.;Krestel Heinz E, Mihaljevic André L A, Hoffman Dax A, Schneider Armin, ;2004;Neurobiology of disease;17;310-8; 15474368
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Karolinska Institutet, Stockholm, Sweden
Animals used for archiving	wild-type C57BL/6N females
Stage of embryos	2-cell

Literature references

A GFP-equipped bidirectional expression module well suited for monitoring tetracycline-regulated gene expression in mouse.;Krestel H E, Mayford M, Seeburg P H, Sprengel R, ;2001;Nucleic acids research;29;E39; 11266574
Neuronal co-expression of EGFP and beta-galactosidase in mice causes neuropathology and premature death.;Krestel Heinz E, Mihaljevic André L A, Hoffman Dax A, Schneider Armin, ;2004;Neurobiology of disease;17;310-8; 15474368

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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