B6;D-Tg(tetO-lacZ,-GFP/Gria2)1Rsp/Kctt

Status

Available to order

EMMA IDEM:09283
International strain nameB6;D-Tg(tetO-lacZ,-GFP/Gria2)1Rsp/Kctt
Alternative nameTgTetOGFP-GluA2/1 (labname TgOCN1)
Strain typeTransgenic Strains
Allele/Transgene symbolTg(tetO-lacZ,-GFP/Gria2)1Rsp
Gene/Transgene symbolTg(tetO-lacZ,-GFP/Gria2)1Rsp

Information from provider

ProviderRolf Sprengel
Provider affiliationMolecular Neurobiology, Max Planck Institute for Medical Research
Genetic informationA transgene for the N-terminal GFP tagged AMPA receptor subunit GluA2 (Gria2), together with a nuclear localised lacZ reporter gene, is under the control of the tTA-dependent bidirectional synthetic promoter. Mouse lines TgTetOGFP-GluA2/1 and TgTetOGFP-GluA2/6 are derived from two different founders from the same pronucleus injection.
Phenotypic informationHomozygous:
Not known

Heterozygous:
When activated by tTA the GFP-GluA2 transgene can partially rescue the GluA2 knock-out (Gria2-/-) mice.
Breeding historyEmbryos from matings of hemizygote transgenes TgTet0GFP-GluA2 males and C57BL/6N females were harvested and 93 embryos were cryoconserved in January 2003
References
  • Enhanced odor discrimination and impaired olfactory memory by spatially controlled switch of AMPA receptors.;Shimshek Derya R, Bus Thorsten, Kim Jinhyun, Mihaljevic Andre, Mack Volker, Seeburg Peter H, Sprengel Rolf, Schaefer Andreas T, ;2005;PLoS biology;3;e354; 16216087
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden
Animals used for archivingheterozygous C57BL/6N, wild-type C57BL/6N
Stage of embryos2-cell

Literature references

  • Enhanced odor discrimination and impaired olfactory memory by spatially controlled switch of AMPA receptors.;Shimshek Derya R, Bus Thorsten, Kim Jinhyun, Mihaljevic Andre, Mack Volker, Seeburg Peter H, Sprengel Rolf, Schaefer Andreas T, ;2005;PLoS biology;3;e354; 16216087

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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