C57BL/6NTac-Pgam5tm1a(EUCOMM)Wtsi/IcsOrl

Status

Available to order

EMMA IDEM:09258
International strain nameC57BL/6NTac-Pgam5tm1a(EUCOMM)Wtsi/IcsOrl
Alternative nameEPD0226_5_A02
Strain typeTargeted Mutant Strains
Allele/Transgene symbolPgam5tm1a(EUCOMM)Wtsi
Gene/Transgene symbolPgam5
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider ICS, Institut Clinique de la Souris
Provider affiliationICS, Institut Clinique de la Souris
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0226_5_A02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous C57BL/6NTac

Disease and phenotype information

IMPC phenotypes (gene matching)
  • increased circulating creatinine level / IMPC
  • decreased circulating cholesterol level / IMPC
  • decreased circulating total protein level / IMPC
  • decreased circulating serum albumin level / IMPC
  • decreased circulating glucose level / IMPC
  • increased mean corpuscular volume / IMPC
  • decreased total retina thickness / IMPC
  • short tibia / IMPC
  • decreased exploration in new environment / IMPC
  • decreased circulating HDL cholesterol level / IMPC
  • decreased erythrocyte cell number / IMPC
MGI phenotypes (allele matching)
  • abnormal gait / MGI
  • short stride length / MGI
  • abnormal motor coordination/ balance / MGI
  • akinesia / MGI
  • decreased vertical activity / MGI
  • no phenotypic analysis / MGI
  • neuron degeneration / MGI
  • loss of dopaminergic neurons / MGI
  • oxidative stress / MGI
  • bradykinesia / MGI
  • homeostasis/metabolism phenotype / MGI
  • decreased dopamine level / MGI
  • abnormal mitochondrion morphology / MGI
  • abnormal mitochondrial physiology / MGI
  • abnormal mitochondrial crista morphology / MGI
MGI phenotypes (gene matching)
  • decreased body weight / MGI
  • decreased body size / MGI
  • abnormal gait / MGI
  • short stride length / MGI
  • abnormal motor coordination/balance / MGI
  • premature death / MGI
  • no abnormal phenotype detected / MGI
  • akinesia / MGI
  • decreased vertical activity / MGI
  • no phenotypic analysis / MGI
  • abnormal redox activity / MGI
  • neuron degeneration / MGI
  • loss of dopaminergic neurons / MGI
  • oxidative stress / MGI
  • bradykinesia / MGI
  • homeostasis/metabolism phenotype / MGI
  • cellular phenotype / MGI
  • decreased dopamine level / MGI
  • abnormal mitochondrion morphology / MGI
  • abnormal mitochondrial physiology / MGI
  • abnormal interleukin-1 beta secretion / MGI
  • decreased interleukin-1 beta secretion / MGI
  • decreased survivor rate / MGI
  • abnormal macrophage activation involved in immune response / MGI
  • prenatal lethality, incomplete penetrance / MGI
  • abnormal mitochondrial shape / MGI
  • abnormal mitochondrial crista morphology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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