STOCK Tg(Camk2a-Grin2c/itTA)12Rsp Tg(tetO-cre)LC1Bjd/Kctt

Status	Available to order
EMMA ID	EM:09256
International strain name	STOCK Tg(Camk2a-Grin2c/itTA)12Rsp Tg(tetO-cre)LC1Bjd/Kctt
Alternative name	TgCN12+LC1 (lab name CN12)
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Camk2a-Grin2c/itTA)12Rsp, Tg(tetO-cre)LC1Bjd
Gene/Transgene symbol	Tg(Camk2a-Grin2c/itTA)12Rsp, Tg(tetO-cre)LC1Bjd

Information from provider

Provider	Rolf Sprengel
Provider affiliation	Molecular Neurobiology, Max Planck Institute for Medicarl Reasearch
Genetic information	The transgene CN12 drives the expression of the humanized version of the tTA (itTA) by CamkII promoter and the neuron specific silencer of the grin2C gene specific to excitatory neurons in the forebrain. The transgene LC encodes the tTA dependent luciferase and Cre recombinase under the control of the bidirectional TetO promoter.
Phenotypic information	Homozygous: not known Heterozygous: In the presence of doxycycline in the drinking water the transgenes are not expressed
Breeding history	70 embryos from matings of double transgenic mice with C57BL/6 mice were harvested and cryoconserved in March 2003
References	Dissecting spatial knowledge from spatial choice by hippocampal NMDA receptor deletion.;Bannerman David M, Bus Thorsten, Taylor Amy, Sanderson David J, Schwarz Inna, Jensen Vidar, Hvalby Øivind, Rawlins J Nicholas P, Seeburg Peter H, Sprengel Rolf, ;2012;Nature neuroscience;15;1153-9; 22797694 Stringent doxycycline dependent control of CRE recombinase in vivo.;Schönig Kai, Schwenk Frieder, Rajewsky Klaus, Bujard Hermann, ;2002;Nucleic acids research;30;e134; 12466566 Age-Dependent Degeneration of Mature Dentate Gyrus Granule Cells Following NMDA Receptor Ablation.;Watanabe Yasuhito, Müller Michaela K, von Engelhardt Jakob, Sprengel Rolf, Seeburg Peter H, Monyer Hannah, ;2015;Frontiers in molecular neuroscience;8;87; 26793056
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Karolinska Institutet, Stockholm, Sweden
Animals used for archiving	heterozygous 0, wild-type 0
Stage of embryos	2-cell

Literature references

Dissecting spatial knowledge from spatial choice by hippocampal NMDA receptor deletion.;Bannerman David M, Bus Thorsten, Taylor Amy, Sanderson David J, Schwarz Inna, Jensen Vidar, Hvalby Øivind, Rawlins J Nicholas P, Seeburg Peter H, Sprengel Rolf, ;2012;Nature neuroscience;15;1153-9; 22797694
Stringent doxycycline dependent control of CRE recombinase in vivo.;Schönig Kai, Schwenk Frieder, Rajewsky Klaus, Bujard Hermann, ;2002;Nucleic acids research;30;e134; 12466566
Age-Dependent Degeneration of Mature Dentate Gyrus Granule Cells Following NMDA Receptor Ablation.;Watanabe Yasuhito, Müller Michaela K, von Engelhardt Jakob, Sprengel Rolf, Seeburg Peter H, Monyer Hannah, ;2015;Frontiers in molecular neuroscience;8;87; 26793056

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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