C57BL/6N-Smc6^{tm1b(EUCOMM)Hmgu}/Ieg

Status	Available to order
EMMA ID	EM:09249
International strain name	C57BL/6N-Smc6^{tm1b(EUCOMM)Hmgu}/Ieg
Alternative name	HEPD0531_1_D05
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Smc6^{tm1b(EUCOMM)Hmgu}
Gene/Transgene symbol	Smc6
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0531_1_D05. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sortm1(ACTB-cre,-EGFP)Ics (MGI:5285392)). Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6NTac

Disease and phenotype information

IMPC phenotypes (allele matching)

increased circulating serum albumin level / IMPC
decreased lean body mass / IMPC
increased total body fat amount / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, complete penetrance / IMPC
increased total body fat amount / IMPC
increased circulating serum albumin level / IMPC
decreased lean body mass / IMPC

MGI phenotypes (gene matching)

decreased circulating HDL cholesterol level / MGI
increased circulating calcium level / MGI
increased leukocyte cell number / MGI
macrocytosis / MGI
decreased body weight / MGI
decreased body size / MGI
abnormal motor coordination/balance / MGI
decreased embryo size / MGI
decreased litter size / MGI
increased thermal nociceptive threshold / MGI
embryonic growth retardation / MGI
decreased fetal size / MGI
growth/size/body region phenotype / MGI
thrombocytosis / MGI
increased circulating glucose level / MGI
decreased body mass index / MGI
decreased IgG2b level / MGI
decreased total body fat amount / MGI
mortality/aging / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
preweaning lethality, incomplete penetrance / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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