C57BL/6N-Pkn2^{tm1b(KOMP)Wtsi}/Ieg

Status	Available to order
EMMA ID	EM:09244
International strain name	C57BL/6N-Pkn2^{tm1b(KOMP)Wtsi}/Ieg
Alternative name	EPD0319_7_G05
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Pkn2^{tm1b(KOMP)Wtsi}
Gene/Transgene symbol	Pkn2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from KOMP ES clone EPD0319_7_G05. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sortm1(ACTB-cre,-EGFP)Ics (MGI:5285392)). Click here for more information on KOMP final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6NTac

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased circulating HDL cholesterol level / IMPC
decreased exploration in new environment / IMPC
increased startle reflex / IMPC
decreased circulating cholesterol level / IMPC
increased circulating bilirubin level / IMPC
decreased circulating total protein level / IMPC
preweaning lethality, complete penetrance / IMPC
decreased circulating unsaturated transferrin level / IMPC

IMPC phenotypes (gene matching)

decreased circulating total protein level / IMPC
increased circulating bilirubin level / IMPC
decreased circulating HDL cholesterol level / IMPC
decreased exploration in new environment / IMPC
decreased circulating unsaturated transferrin level / IMPC
decreased circulating cholesterol level / IMPC
increased startle reflex / IMPC
preweaning lethality, complete penetrance / IMPC

MGI phenotypes (gene matching)

abnormal vascular development / MGI
open neural tube / MGI
abnormal somite development / MGI
abnormal embryo turning / MGI
absent vitelline blood vessels / MGI
disorganized yolk sac vascular plexus / MGI
pericardial edema / MGI
abnormal developmental patterning / MGI
abnormal neural tube morphology / MGI
abnormal neural crest cell migration / MGI
abnormal vitelline vasculature morphology / MGI
absent pharyngeal arches / MGI
abnormal rostral-caudal body axis extension / MGI
abnormal neural fold formation / MGI
abnormal mesenchyme morphology / MGI
craniorachischisis / MGI
delayed heart looping / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
abnormal fibroblast proliferation / MGI
abnormal head development / MGI
abnormal vascular plexus formation / MGI
abnormal germ layer morphology / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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