C57BL/6N-Hoxa2tm1b(EUCOMM)Wtsi/Ieg

Status

Available to order

EMMA IDEM:09237
International strain nameC57BL/6N-Hoxa2tm1b(EUCOMM)Wtsi/Ieg
Alternative nameEPD0560_3_F04
Strain typeTargeted Mutant Strains
Allele/Transgene symbolHoxa2tm1b(EUCOMM)Wtsi
Gene/Transgene symbolHoxa2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliationInstitute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0560_3_F04. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sortm1(ACTB-cre,-EGFP)Ics (MGI:5285392)). Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6NTac

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • abnormal snout morphology / IMPC
  • abnormal gait / IMPC
  • abnormal auditory brainstem response / IMPC
  • improved glucose tolerance / IMPC
  • decreased circulating creatinine level / IMPC
  • decreased prepulse inhibition / IMPC
  • decreased respiratory quotient / IMPC
  • preweaning lethality, complete penetrance / IMPC
IMPC phenotypes (gene matching)
  • decreased circulating creatinine level / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • decreased prepulse inhibition / IMPC
  • abnormal auditory brainstem response / IMPC
  • abnormal gait / IMPC
  • improved glucose tolerance / IMPC
  • abnormal snout morphology / IMPC
  • decreased respiratory quotient / IMPC
MGI phenotypes (gene matching)
  • abnormal malleus morphology / MGI
  • abnormal tympanic ring morphology / MGI
  • abnormal otic capsule morphology / MGI
  • abnormal basioccipital bone morphology / MGI
  • abnormal basisphenoid bone morphology / MGI
  • cleft palate / MGI
  • abnormal cartilage development / MGI
  • abnormal craniofacial morphology / MGI
  • abnormal cranium morphology / MGI
  • abnormal facial motor nucleus morphology / MGI
  • abnormal brain development / MGI
  • abnormal rhombomere morphology / MGI
  • decreased sensory neuron number / MGI
  • abnormal facial nerve morphology / MGI
  • abnormal glossopharyngeal nerve morphology / MGI
  • abnormal vagus nerve morphology / MGI
  • abnormal suckling behavior / MGI
  • aphagia / MGI
  • postnatal growth retardation / MGI
  • abnormal ear morphology / MGI
  • abnormal skeleton development / MGI
  • abnormal craniofacial bone morphology / MGI
  • no abnormal phenotype detected / MGI
  • abnormal outer ear morphology / MGI
  • abnormal hyoid bone morphology / MGI
  • absent malleus processus brevis / MGI
  • abnormal scala vestibuli morphology / MGI
  • nervous system phenotype / MGI
  • abnormal nervous system morphology / MGI
  • abnormal tongue muscle morphology / MGI
  • absent stapes / MGI
  • abnormal temporal bone squamous part morphology / MGI
  • abnormal stapedial artery morphology / MGI
  • absent stapedial artery / MGI
  • abnormal behavior / MGI
  • abnormal middle ear ossicle morphology / MGI
  • abnormal incus morphology / MGI
  • abnormal stapes morphology / MGI
  • abnormal temporal bone morphology / MGI
  • hearing/vestibular/ear phenotype / MGI
  • abnormal skeleton morphology / MGI
  • absent outer ear / MGI
  • abnormal Meckel's cartilage morphology / MGI
  • abnormal tympanic membrane morphology / MGI
  • abnormal external auditory canal morphology / MGI
  • abnormal styloid process morphology / MGI
  • small malleus processus brevis / MGI
  • abnormal gonial bone morphology / MGI
  • enlarged gonial bone / MGI
  • absent styloid process / MGI
  • meteorism / MGI
  • cleft secondary palate / MGI
  • bifid tongue / MGI
  • abnormal hyoid bone lesser horn morphology / MGI
  • absent hyoid bone lesser horns / MGI
  • abnormal neuron differentiation / MGI
  • increased oligodendrocyte progenitor number / MGI
  • lethality at weaning, complete penetrance / MGI
  • neonatal lethality, complete penetrance / MGI
  • increased rhombomere 1 size / MGI
  • abnormal rhombomere 2 morphology / MGI
  • abnormal rhombomere 3 morphology / MGI
  • decreased rhombomere 3 size / MGI
  • abnormal rhombomere boundary morphology / MGI
  • abnormal incudostapedial joint morphology / MGI
  • abnormal retrotympanic process morphology / MGI
  • absent stylohyoid muscle / MGI
  • abnormal tympanic cavity morphology / MGI
  • absent stapedius muscle / MGI
  • abnormal tensor tympani muscle morphology / MGI
  • abnormal styloglossus muscle morphology / MGI
  • abnormal tubotympanic recess morphology / MGI
  • abnormal temporal bone tympanic part morphology / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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